Institute for Translational Medicine, Qingdao University, Qingdao, China.
The Affiliated Hospital of Qingdao University, Qingdao, China.
J Cell Mol Med. 2020 Jan;24(1):1146-1150. doi: 10.1111/jcmm.14802. Epub 2019 Nov 11.
Coronary artery disease (CAD) is one of the biggest threats to human life. Circulating microRNAs (miRNAs) have been reported to be linked to the pathogenesis of CAD, indicating the possible role in CAD diagnosis. The present study aimed to explore the expression profile of plasma miRNAs and estimate their value in diagnosis for CAD. 67 Non-CAD control subjects and 88 CAD patients were enrolled. We conducted careful evaluation by RT-PCR analysis, Spearman rank correlation coefficients analysis, Receiver Operating Characteristic (ROC) curves analysis and so on. The plasma levels of six miRNAs known to be related to CAD were measured and three of them showed obvious expression change. Circulating miR-29a-3p, miR-574-3p and miR-574-5p were all significantly increased. ROC analysis revealed the probability of the three miRNAs as biomarkers with AUCs (areas under the ROC curve) of 0.830, 0.792 and 0.789, respectively. They were significantly correlated with each other in CAD patients, suggesting the possibility of joint diagnosis. The combined AUC was 0.915, much higher than each single miRNA. Therefore, our study revealed three promising biomarkers for early diagnosis of CAD. The combination of these miRNAs may act more effectively than individual ones for CAD diagnosis.
冠状动脉疾病（CAD）是对人类生命最大的威胁之一。循环microRNAs（miRNAs）已被报道与 CAD 的发病机制有关，表明其在 CAD 诊断中可能具有一定的作用。本研究旨在探索血浆 miRNAs 的表达谱，并评估其在 CAD 诊断中的价值。纳入 67 名非 CAD 对照组和 88 名 CAD 患者。我们通过 RT-PCR 分析、Spearman 秩相关系数分析、接收者操作特征（ROC）曲线分析等进行了仔细评估。测量了已知与 CAD 相关的六种 miRNA 的血浆水平，其中三种显示出明显的表达变化。循环 miR-29a-3p、miR-574-3p 和 miR-574-5p 均显著升高。ROC 分析显示，这三个 miRNA 作为生物标志物的概率具有 0.830、0.792 和 0.789 的 AUC（ROC 曲线下的面积）。它们在 CAD 患者中彼此显著相关，表明联合诊断的可能性。联合 AUC 为 0.915，明显高于单个 miRNA。因此，我们的研究揭示了三个有希望的 CAD 早期诊断的生物标志物。这些 miRNA 的组合可能比单个 miRNA 对 CAD 诊断更有效。
