Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Tianjin, China.
Nankai University, Tianjin, China.
J Cardiovasc Transl Res. 2020 Apr;13(2):181-190. doi: 10.1007/s12265-019-09925-x. Epub 2019 Nov 11.
The long-term patency rate of saphenous vein (SV) grafts is poor compared to arterial grafts. To investigate the effects of surgical preparation (distention) of SV on hydrogen sulfide (HS) released from the endothelium, human SV segments were harvested from 43 patients during coronary artery bypass surgery (CABG). Acetylcholine (ACh) induced relaxation that was inhibited by N-nitro-L-arginine + indomethacin and cysteine aminotransferase inhibitor aminooxyacetic acid in the normal SV. In contrast, ACh did not evoke relaxation in the distended SV (DSV). The concentration of HS quantified by methylene blue assay in DSV was significantly lower than that in control. Transmission electron microscope and immunohistochemistry studies showed that the preparation destroyed the endothelium, smooth muscle, organelle, and vasa vasorum. We conclude that surgical preparation injures the endothelium and smooth muscle of the SV grafts and reduces HS release from SV. These effects may contribute to the poor long-term patency of the SV graft.
与动脉移植物相比，大隐静脉（SV）移植物的长期通畅率较差。为了研究 SV 内皮细胞释放的硫化氢（HS）的手术准备（扩张）的影响，在冠状动脉旁路移植术（CABG）期间从 43 名患者中采集了人类 SV 段。乙酰胆碱（ACh）诱导的松弛被 N-硝基-L-精氨酸+吲哚美辛和半胱氨酸氨基转移酶抑制剂氨基氧乙酸抑制，在正常的 SV 中。相比之下，扩张的 SV（DSV）中 ACh 不能引起松弛。亚甲基蓝法测定的 DSV 中 HS 的浓度明显低于对照。透射电镜和免疫组织化学研究表明，该制剂破坏了 SV 移植物的内皮细胞、平滑肌、细胞器和血管腔。我们得出结论，手术准备会损伤 SV 移植物的内皮细胞和平滑肌，并减少 SV 中 HS 的释放。这些影响可能导致 SV 移植物的长期通畅率较差。
