Key Lab of Urban Environment and Health , Institute of Urban Environment, Chinese Academy of Sciences , Xiamen 361021 , China.
State Key Laboratory of Urban and Regional Ecology , Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085 , China.
Anal Chem. 2019 Dec 17;91(24):15345-15354. doi: 10.1021/acs.analchem.9b03996. Epub 2019 Nov 25.
With the ever-increasing demand for improved medical diagnosis, safe food supply, advanced biotechnology, and sustainable ecosystems, characterization of the microbial world by sensitive, specific, rapid, and quantitative tools is gaining more and more attentions. Surface-enhanced Raman spectroscopy (SERS) and its flexible integration with other tools, such as microscopes, stable isotope probing, microfluidic analysis, and chemometric analysis, have advanced rapidly and showed great promise for versatile microbial characterization. This perspective provides an overview of the recent advances of SERS and related coupling techniques for microbial diagnosis (e.g., identification and antibiotic resistance testing), phenotypic response profiling, microbial function assessment, in situ biofilm characterization, and multifunctional SERS tags. We further propose future requirements and the direction for SERS methodology to be headed, including standardization of SERS methodology, database generation and management, coupling with omics technology and single-cell sorting, and application in deciphering microbial processes.
随着人们对改进医学诊断、安全食品供应、先进生物技术和可持续生态系统的需求不断增加，通过敏感、特异、快速和定量的工具来描述微生物世界的特性越来越受到关注。表面增强拉曼光谱（SERS）及其与显微镜、稳定同位素探测、微流控分析和化学计量学分析等其他工具的灵活集成，得到了快速发展，并为多功能微生物特性描述提供了广阔的前景。本文综述了 SERS 及其相关耦合技术在微生物诊断（例如，鉴定和抗生素耐药性测试）、表型反应谱分析、微生物功能评估、原位生物膜特性和多功能 SERS 标签方面的最新进展。我们进一步提出了未来对 SERS 方法学的要求和发展方向，包括 SERS 方法学的标准化、数据库的生成和管理、与组学技术和单细胞分选的耦合，以及在破译微生物过程中的应用。
