Department of Preclinical Medicine, Faculty of Veterinary Medicine, University of Kinshasa, Kinshasa XI.
Onderstepoort J Vet Res. 2019 Oct 10;86(1):e1-e5. doi: 10.4102/ojvr.v86i1.1737.
Rift Valley fever (RVF) is a zoonotic viral disease caused by the RVF phlebovirus (RVFV) that infects a variety of animal species including sheep and goats. Sera (n = 893) collected between 2013 and 2015 from randomly selected indigenous sheep and goats in seven provinces of the Democratic Republic of the Congo (DRC) were tested for the presence of specific immunoglobulin G (IgG) and M (IgM) against RVFV, using two commercially available enzyme-linked immunosorbent assays. The reverse transcription polymerase chain reaction (RT-PCR) was also used to detect RVFV nucleic acid. There was significant variation in true seroprevalence of RVFV for both sheep and goats between the seven provinces investigated. Values ranged from 0.0 (95% confidence interval [CI] 0.0-6.55) to 23.81 (95% CI 12.03-41.76) for goat and 0.0 (95% CI 0.0-7.56) to 37.11 (95% CI 15.48-65.94) for sheep, respectively. One serum (1.85%) out of 54 that tested positive for IgG was found to be IgM-positive. This same sample was also positive by RT-PCR indicating an active or recent infection. These findings report the presence of RVFV in small ruminants in the DRC for the first time and indicate variations in exposure to the virus in different parts of the country.
裂谷热（RVF）是一种由裂谷热病毒（RVFV）引起的人畜共患病，可感染绵羊和山羊等多种动物。2013 年至 2015 年期间，从刚果民主共和国（DRC）7 个省份随机选择的本地绵羊和山羊采集了 893 份血清，使用两种市售的酶联免疫吸附试验（ELISA）检测针对 RVFV 的特异性 IgG 和 IgM。还使用逆转录聚合酶链反应（RT-PCR）检测 RVFV 核酸。在所调查的 7 个省份中，绵羊和山羊的 RVFV 真实血清阳性率存在显著差异。山羊的数值范围为 0.0（95%置信区间[CI] 0.0-6.55）至 23.81（95% CI 12.03-41.76），绵羊的数值范围为 0.0（95% CI 0.0-7.56）至 37.11（95% CI 15.48-65.94）。54 份 IgG 检测呈阳性的血清中，有 1 份（1.85%）被发现 IgM 呈阳性。该样本也通过 RT-PCR 呈阳性，表明存在活跃或近期感染。这些发现首次报告了刚果民主共和国小反刍动物中存在 RVFV，并表明该国不同地区对该病毒的暴露存在差异。
