Edström A, Hanson M, Wallin M, Cederholm B
Acta Physiol Scand. 1979 Nov;107(3):233-37. doi: 10.1111/j.1748-1716.1979.tb06468.x.
The effects of colchicine and colchiceine on fast axonal transport in frog sciatic nerves were studied in vitro. Colchiceine inhibited the transport to about the same extent as colchicine. Preincubation at low temperature potentiated the inhibitory effect of either drug. The polymerization of purified brain tubulin was inhibited by colchiceine at 5-10 times higher concentrations than colchicine. The similarity of the effects obtained with colchicine and colchiceine indicates that both drugs arrest axonal transport by interfering with microtubule function. Colchicine and colchiceine did not affect the levels of high energy phosphates (ATP and CrP) in frog nerves indicating that a reduced energy supply was not responsible for the arrested transport.
在体外研究了秋水仙碱和秋水仙酰胺对青蛙坐骨神经快速轴突运输的影响。秋水仙酰胺对运输的抑制程度与秋水仙碱大致相同。低温预孵育增强了两种药物的抑制作用。纯化的脑微管蛋白的聚合被秋水仙酰胺抑制的浓度比秋水仙碱高5至10倍。秋水仙碱和秋水仙酰胺所产生的效应的相似性表明，两种药物都是通过干扰微管功能来阻止轴突运输的。秋水仙碱和秋水仙酰胺不影响青蛙神经中高能磷酸盐（ATP和CrP）的水平，这表明能量供应减少不是运输受阻的原因。
