Shibata H, Hara H
Department of Veterinary Pharmacology, Faculty of Agriculture, Yamaguchi University, Japan.
J Toxicol Sci. 1988 May;13(2):83-96. doi: 10.2131/jts.13.83.
Organ distribution pattern, hepatic uptake ratio, and blood clearance were examined by giving 99mTc-phytate (99mTc-P) to the normal rats. At the same time, the relation between the severity of hepatic function and blood clearance or hepatic uptake ratio of 99mTc-P was studied by using the rats with acute hepatic dysfunction experimentally induced by carbon tetrachloride (CCl4) administration. Furthermore, a comparative discussion on blood clearance test of 99mTc-P was made with serum transaminase test and with histological test of the liver. It appeared appropriate to administer 99mTc-P at the dose of 500 micrograms/kg in order to obtain an effective blood clearance curve. A major part of 99mTc-P intravenously administered was take up into the liver, while the remainder of small amount into the spleen, kidneys, lung, and so on. Little was recognized in the thyroid gland. The hepatic uptake ratio of 99mTc-P reached the maximum 15 minutes after the administration. The disappearance rate of 99mTc-P from blood decreased with the increase in dose of CCl4 and with the passage of time after the CCl4 administration. The blood clearance test of 99mTc-P showed a sensitive reaction for the acute hepatic dysfunction induced by CCl4 equally to or higher than the serum transaminase test or histological test of the liver.
通过给正常大鼠注射99m锝-植酸盐（99mTc-P）来检测器官分布模式、肝脏摄取率和血液清除率。同时，利用通过给予四氯化碳（CCl4）实验性诱导急性肝功能障碍的大鼠，研究肝功能严重程度与99mTc-P血液清除率或肝脏摄取率之间的关系。此外，对99mTc-P的血液清除试验与血清转氨酶试验以及肝脏组织学试验进行了比较讨论。为了获得有效的血液清除曲线，以500微克/千克的剂量注射99mTc-P似乎是合适的。静脉注射的99mTc-P大部分被肝脏摄取，而其余少量被脾脏、肾脏、肺等摄取。甲状腺中几乎没有发现。99mTc-P的肝脏摄取率在给药后15分钟达到最大值。随着CCl4剂量的增加以及CCl4给药后时间的推移，99mTc-P从血液中的消失率降低。99mTc-P的血液清除试验对CCl4诱导的急性肝功能障碍显示出与血清转氨酶试验或肝脏组织学试验相同或更高的敏感反应。
