School of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bently, WA, 6102, Australia.
Department of Fisheries Biology & Genetics, Faculty of Fisheries, Patuakhali Science and Technology University, Patuakhali, 8602, Bangladesh.
Sci Rep. 2019 Nov 13;9(1):16703. doi: 10.1038/s41598-019-53018-3.
This study investigated the effects of replacement of fishmeal (FM) with poultry by-product (PBM) protein, supplemented with black soldier fly, Hermetia illucens (HI) larvae on growth, histomormhology, immunity and resistance to Vibrio harveyi in juvenile barramundi. Two hundred and twenty five barramundi averaging 3.51 ± 0.03 g were randomly allocated into three groups and fed isonitrogenous and isocalorific diets containing different levels of PBM supplemented with HI as follows: Control (FM based diet), 45PBM + HI (45% PBM supplemented with 10% HI), and 90PBM + HI (90% PBM supplemented with 10% HI) for 6 weeks. Results showed that dietary inclusion of 45PBM + HI significantly improved the growth performance than control whereas growth inhibition occurred in the 90PBM + HI. The 45PBM + HI groups demonstrated significant increases in histometric measurements (villus and enterocyte width, and microvilli height) and acidic mucins. The impaired growth in 90PBM + HI groups was further associated with multifocal necrosis in the liver, an upregulation of the stress related genes (HSP70 and HSP90) and increase in the levels of liver enzymes. When 45PBM + HI was fed, survival against V. harveyi increased significantly and also an increase in serum immunity and immune-related genes in the head kidney was observed after infection.
本研究调查了用家禽副产品（PBM）蛋白替代鱼粉（FM），并补充黑水虻（Hermetia illucens，HI）幼虫对军曹鱼幼鱼生长、组织形态学、免疫和抗哈维弧菌的影响。225 尾平均体重为 3.51±0.03g 的军曹鱼被随机分配到 3 组，并分别投喂含有不同水平 PBM 并补充 HI 的等氮等热量饮食，具体如下：对照组（基于 FM 的饮食）、45PBM+HI（45% PBM 补充 10% HI）和 90PBM+HI（90% PBM 补充 10% HI），为期 6 周。结果表明，与对照组相比，饲料中添加 45PBM+HI 可显著提高生长性能，而 90PBM+HI 则抑制了生长。45PBM+HI 组的组织学测量（绒毛和肠细胞宽度以及微绒毛高度）和酸性粘蛋白显著增加。90PBM+HI 组的生长受损与肝脏的多灶性坏死有关，还与应激相关基因（HSP70 和 HSP90）的上调和肝脏酶水平的升高有关。当投喂 45PBM+HI 时，对哈维弧菌的存活率显著提高，感染后头肾的血清免疫和免疫相关基因也增加。
