School of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA, 6102, Australia.
School of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA, 6102, Australia.
Fish Shellfish Immunol. 2020 Sep;104:567-578. doi: 10.1016/j.fsi.2020.06.014. Epub 2020 Jun 18.
In an effort to reduce the use of fishmeal (FM), the effect of using protein from poultry by product meal (PBM) along with the supplementation of three different fish protein hydrolysate (FPH) including yellowtail kingfish, carp and tuna hydrolysate (designated as KH, CH and TH, respectively) were evaluated in juvenile barramundi for growth performance, fillet quality, mucosal immunity, serum biochemistry, immune response and infection against Vibrio harveyi. Fish were fed a FM based control diet + three isonitrogenous and isolipidic diets containing 90% of PBM protein supplemented with different types of hydrolysates: 90% PBM +10% KH (90PBM + KH), 90% PBM + 10% CH (90PBM + CH) and 90% PBM + 10% TH (90PBM + TH). Growth performance and indices were unaffected by the hydrolysate supplemented diets when compared to the control. FPH supplemented PBM diets resulted in improved muscle quality by improving poly unsaturated fatty acids (PUFA), ∑n-3, ∑n-6 and ∑n-9, and health related lipid indexes were not affected. The internal architecture of spleen and kidney were not altered by test diets whilst FPH supplemented PBM modulated acidic mucins in intestine and skin of fish. Improved infection rate in response to two weeks post infection with V. harveyi in the FPH supplemented diets was further associated with an increased serum immune response and a concomitant regulation of proinflammatory and inflammatory cytokines in the head kidney. Serum biochemistry including alanine transaminase (ALT), glutamate dehydrogenase (GLDH) and total bilirubin (TB) showed a decreasing trend both in pre-challenge and post-challenge barramundi fed FPH supplemented diets whereas cholesterol level decreased significantly in post-challenge groups fed 90PBM + KH and 90PBM + TH than pre-challenge barramundi. This study signifies that supplementation of 10% with different three FPH, hydrolysed by an alcalase® enzyme in PBM-based diets for barramundi could be good strategies to overcome the negative consequences triggered by animal by-product ingredients.
为了减少鱼粉（FM）的使用，本研究评估了在幼鲈中使用禽副产品粉（PBM）蛋白，以及补充三种不同的鱼蛋白水解物（FPH），包括黄鳍金枪鱼、鲤鱼和金枪鱼水解物（分别指定为 KH、CH 和 TH）对生长性能、鱼片质量、黏膜免疫、血清生化、免疫反应和感染哈维弧菌的影响。鱼被喂食基于 FM 的对照饮食+三种等氮和等脂的饮食，含有 90%的 PBM 蛋白，补充不同类型的水解物：90% PBM+10% KH（90PBM+KH）、90% PBM+10% CH（90PBM+CH）和 90% PBM+10% TH（90PBM+TH）。与对照组相比，补充水解物的饮食对生长性能和指标没有影响。FPH 补充 PBM 饮食通过改善多不饱和脂肪酸（PUFA）、∑n-3、∑n-6 和∑n-9，改善了肌肉质量，并且健康相关的脂质指标不受影响。测试饮食未改变脾脏和肾脏的内部结构，而 FPH 补充 PBM 调节了鱼肠和皮肤中的酸性粘蛋白。在 FPH 补充饮食中，两周后感染哈维弧菌的感染率提高，与血清免疫反应增加以及头肾中促炎和炎症细胞因子的调节有关。血清生化包括丙氨酸转氨酶（ALT）、谷氨酸脱氢酶（GLDH）和总胆红素（TB）在 FPH 补充饮食喂养的鱼中，无论是在攻毒前还是攻毒后都呈下降趋势，而胆固醇水平在攻毒后 90PBM+KH 和 90PBM+TH 组显著低于攻毒前的 90PBM+KH 和 90PBM+TH 组。本研究表明，在基于 PBM 的饮食中，用碱性蛋白酶水解不同的三种 FPH 补充 10%，可能是克服动物副产品成分引发的负面影响的良好策略。
