Zhou Jing, Tzanetakis Ioannis E
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, USA.
J Gen Virol. 2020 Jan;101(1):112-121. doi: 10.1099/jgv.0.001352.
Orthotospoviruses are acquired by thrips during feeding on infected tissue. Virions travel through the foregut and enter midgut epithelial cells through the interaction between the viral glycoproteins and cellular receptors. Glycoprotein RGD motifs and N-linked glycosylation sites have been predicted to mediate receptor binding or play important roles in virus entry into host cells, yet their function needs to be validated. In this study, peptides derived from the soybean vein necrosis virus N glycoprotein were utilized to identify critical regions in virus-vector interactions. Transmission mediated by single dropped by more than 2/3 when thrips were fed on peptide NASIAAAHEVSQE or the combination of NASIRGDHEVSQE and RLTGECNITKVSLTN when compared to the controls; indicating that this strategy could significantly reduce transmission efficiency, opening new avenues in the control of diseases caused by orthotospoviruses.
正番茄斑萎病毒属病毒在蓟马取食受感染组织时被获取。病毒粒子穿过前肠，并通过病毒糖蛋白与细胞受体之间的相互作用进入中肠上皮细胞。糖蛋白RGD基序和N-连接糖基化位点已被预测可介导受体结合或在病毒进入宿主细胞过程中发挥重要作用，但其功能仍需验证。在本研究中，利用源自大豆脉坏死病毒N糖蛋白的肽来鉴定病毒与载体相互作用中的关键区域。与对照相比，当蓟马取食肽NASIAAAHEVSQE或NASIRGDHEVSQE与RLTGECNITKVSLTN的组合时，单头蓟马介导的传播率下降了2/3以上；这表明该策略可显著降低传播效率，为正番茄斑萎病毒属病毒引起的疾病控制开辟了新途径。
