Ambrosini Adriana, Passaglia Luciane M P
Genetics Department, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Curr Protoc Plant Biol. 2017 Sep;2(3):190-209. doi: 10.1002/pb.20054.
Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth-promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultural inoculants. Agricultural biofertilization and biocontrol of pathogens are eco-friendly alternatives to chemical usage and have less energy, environmental, and economic costs. PGPB isolation and evaluation are essentials steps for determining bacteria that are able to improve plant development and productivity. In this unit, we present protocols to isolate bacteria from soil and plant roots ("putative" diazotrophic and endospore-forming bacteria), as well to evaluate some of their beneficial characteristics for the promotion of plant growth (e.g., nitrogen fixation, production of indolic compounds and siderophores, phosphate solubilization, and 1-aminocyclopropane-1-carboxylate deaminase activity). © 2017 by John Wiley & Sons, Inc.
植物根系与众多不同类型的有益和致病微生物相关联。促进植物生长的（根际）细菌（PGPB或PGPR）在世界各地的农作物中分离得到，其中许多被用作农业接种剂。农业生物施肥和病原体生物防治是化学物质使用的环保替代方法，且能源、环境和经济成本较低。PGPB的分离和评估是确定能够改善植物发育和生产力的细菌的关键步骤。在本单元中，我们介绍了从土壤和植物根系中分离细菌（“假定的”固氮和产芽孢细菌）的方案，以及评估它们促进植物生长的一些有益特性（例如固氮、吲哚类化合物和铁载体的产生、磷溶解以及1-氨基环丙烷-1-羧酸脱氨酶活性）的方案。© 2017约翰威立父子公司版权所有。
