Department of Orthodontics and Craniofacial Developmental Biology, Division of Dental Sciences, Biomedical Sciences Major, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
Sci Rep. 2019 Nov 14;9(1):16764. doi: 10.1038/s41598-019-53265-4.
Stem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (hDPSCs) have emerged as attractive cell sources for bone regeneration. However, the specific teeth and the conditions most suitable for stem cell isolation remain unclear. Therefore, the success rate of SHED and hDPSCs isolation, the patient age and remaining root length in deciduous teeth were evaluated. Successful isolation was defined as when the cell culture was maintained up to the third passage without any contamination or other issues. Remaining tooth length was calculated using the root-to-crown ratio from patient X-rays and compared to the norm value from the literature. The overall successful isolation rate of SHED and hDPSCs was 82% and 70%. The average patient ages at extraction of the deciduous teeth and permanent teeth were 11 years and 9 months, and 22 years and 10 months respectively. In the successful SHED group, the average remaining root length of the anterior deciduous teeth was 71.4%, and that of the deciduous molars was 61.4%. Successful isolation appears to be associated with patient age, length of the remaining root, and also mechanical stress and other factors. Tooth selection criteria need to be identified to improve the success rate.
人脱落乳牙（SHED）和人牙髓干细胞（hDPSCs）作为骨再生有吸引力的细胞来源已经出现。然而，具体的牙齿和最适合干细胞分离的条件尚不清楚。因此，评估了 SHED 和 hDPSCs 分离的成功率、患者年龄和乳牙的剩余根长。成功分离的定义是，当细胞培养物在没有任何污染或其他问题的情况下维持到第三代。使用来自患者 X 光片的根冠比来计算剩余牙齿长度，并与文献中的标准值进行比较。SHED 和 hDPSCs 的总成功分离率分别为 82%和 70%。乳牙和恒牙拔除时的平均患者年龄分别为 11 岁和 9 个月，以及 22 岁和 10 个月。在成功的 SHED 组中，前乳牙的平均剩余根长为 71.4%，乳磨牙为 61.4%。成功的分离似乎与患者年龄、剩余根长以及机械应力等因素有关。需要确定牙齿选择标准以提高成功率。
