Hirabae Akira, Kunimatsu Ryo, Yoshimi Yuki, Rikitake Kodai, Ogashira Shintaro, Nakatani Ayaka, Sakata Shuzo, Tanimoto Kotaro
Department of Orthodontics, Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Cells. 2025 Apr 30;14(9):657. doi: 10.3390/cells14090657.
This study aimed to explore how amelogenin can improve stem cells from human exfoliated deciduous teeth (SHED)-based bone regeneration and promote tissue healing as a treatment for critical-sized bone defects. SHED was induced into bone differentiation by using osteogenic differentiation medium. Real-time polymerase chain reaction, alkaline phosphatase (ALP) staining and quantification, and Alizarin Red S staining, as well as calcium and osteocalcin quantification were performed to assess differentiation. On day 18, a significant increase was observed in the expression of , , , , , , , , and . Osteocalcin gene expression continued to increase significantly. ALP activity was significantly higher in the amelogenin-treated group than in the control group on days 7, 10, and 14. On day 14, enhanced ALP staining was observed in the amelogenin-treated group. Calcium and osteocalcin levels were significantly higher in the amelogenin-treated group than in the control group on day 21. This study suggests that combining SHED and amelogenin may be effective for bone regeneration, offering a potential new approach in regenerative medicine.
本研究旨在探索釉原蛋白如何改善基于人脱落乳牙干细胞（SHED）的骨再生，并促进组织愈合，作为治疗临界尺寸骨缺损的一种方法。使用成骨分化培养基将SHED诱导成骨分化。进行实时聚合酶链反应、碱性磷酸酶（ALP）染色及定量、茜素红S染色以及钙和骨钙素定量以评估分化情况。在第18天，观察到[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]的表达显著增加。骨钙素基因表达持续显著增加。在第7天、10天和14天，釉原蛋白处理组的ALP活性显著高于对照组。在第14天，在釉原蛋白处理组中观察到增强的ALP染色。在第21天，釉原蛋白处理组的钙和骨钙素水平显著高于对照组。本研究表明，将SHED和釉原蛋白结合可能对骨再生有效，为再生医学提供了一种潜在的新方法。
