用结晶紫对中性粒细胞的酸性区进行标记。

Labeling Acidic Compartments of Neutrophils with Cresyl Violet.

机构信息

Program in Cell Biology, Hospital for Sick Children, Toronto, ON, Canada.

Department of Biochemistry, University of Toronto, Toronto, ON, Canada.

出版信息

Methods Mol Biol. 2020;2087:207-213. doi: 10.1007/978-1-0716-0154-9_15.

DOI:10.1007/978-1-0716-0154-9_15

PMID:31728993

Abstract

We introduce the acidotropic marker cresyl violet to stain acidic granules in live neutrophils. Cresyl violet is less phototoxic, more photostable, and more cost-effective than other commercially available acidotropic markers. Additionally, it does not photoconvert to fluorescent species of a different color, a limitation of other commonly used acidotropic markers. Staining can be readily detected by fluorescence microscopy or by flow cytometry, and can be used as a readout of degranulation in activated neutrophils.

摘要

我们介绍了嗜酸性标记物 Cresyl 紫，用于对活中性粒细胞中的酸性颗粒进行染色。Cresyl 紫的光毒性更小、光稳定性更好、成本效益更高，优于其他市售的嗜酸性标记物。此外，它不会光转化为不同颜色的荧光物质，这是其他常用嗜酸性标记物的一个局限性。通过荧光显微镜或流式细胞术可以很容易地检测到染色，并且可以作为激活的中性粒细胞脱颗粒的指标。