Alvarez-Buylla A, Ling C Y, Kirn J R
Rockefeller University, New York, NY 10021.
J Neurosci Methods. 1990 Aug;33(2-3):129-33. doi: 10.1016/0165-0270(90)90016-9.
Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy.
神经生物学家广泛使用甲酚紫在明场显微镜下观察尼氏小体。在此，我们描述一种将这种染料用作红色荧光尼氏染色剂的方法。与明场染色技术不同，荧光甲酚紫可与其他荧光染料和示踪剂兼容，如荧光素、荧光金和固蓝。该程序只需对常规明场甲酚染色进行微小修改，最主要的是对染色剂进行稀释。因此，荧光红色甲酚紫操作简单，可能在荧光显微镜中普遍适用。
