Department of Medicinal and Cosmetic Natural Products, Poznan University of Medicinal Sciences, Mazowiecka 33, 60-623 Poznan, Poland.
Laboratory of Environmental Research, Department of Toxicology, Poznan University of Medicinal Sciences, Dojazd 30, 60-631 Poznan.
Molecules. 2019 Nov 15;24(22):4131. doi: 10.3390/molecules24224131.
The presence of dominant active compounds in standardised methanol extract from the leaves of () was examined using HPLC with a diode-array detector. The extract and three dominant parthenolide derivatives were tested with Serotonin Research ELISA for their ability to inhibit the serotonin release from platelets. The antiserotonin effect of the extract was compared with that of parthenolide, a compound with proven antiserotonin and antimigraine effects. This study aimed to evaluate the ability of natural parthenolide derivatives to inhibit serotonin release from platelets. Izospiciformin, stizolin and stizolicin were analysed along with the standardised alcohol extract of leaves, which also contained four other parthenolide derivatives. All the analysed substances were found to inhibit serotonin release from platelets as compared with the control sample, which had 100% of serotonin released. Izospiciformin had the most significant impact (97.98% serotonin release inhibition). The effect of the methanol extract of on the serotonin release inhibition was also statistically significant.
采用二极管阵列检测器的高效液相色谱法检测了标准化甲醇提取物中（）的优势活性化合物的存在。用 Serotonin Research ELISA 测试提取物和三种主要的角蒿内酯衍生物，以评估它们抑制血小板释放 5-羟色胺的能力。将提取物的抗 5-羟色胺作用与已证明具有抗 5-羟色胺和抗偏头痛作用的角蒿内酯化合物进行了比较。本研究旨在评估天然角蒿内酯衍生物抑制血小板释放 5-羟色胺的能力。对 izospiciformin、stizolin 和 stizolicin 以及含有其他四种角蒿内酯衍生物的标准醇提物进行了分析。与释放了 100% 5-羟色胺的对照样品相比，所有分析的物质都被发现能抑制血小板释放 5-羟色胺。izospiciformin 的抑制作用最为显著（97.98% 5-羟色胺释放抑制）。甲醇提取物对角蒿抑制 5-羟色胺释放的作用也具有统计学意义。
