Department of Pathology, Affiliated Hospital of Jining Medical University, Jining, Shandong, China.
Department of Pathology, Jining First People's Hospital, Jining, Shandong, China.
Bosn J Basic Med Sci. 2020 Aug 3;20(3):357-364. doi: 10.17305/bjbms.2019.4408.
Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is a highly conserved long noncoding RNA, which has been related to various pathological processes, including cancer. The role and mechanism of MALAT1 in colon cancer are not clear. We investigated MALAT1 expression in colon cancer tissues, the effect of MALAT1 on proliferation and apoptosis of SW480 cells, and the signaling pathway involved in the MALAT1 effects. MALAT1 expression was determined in 60 colon cancer and para-carcinoma tissues using reverse transcription polymerase chain reaction (RT-PCR). Knockdown of MALAT1 in SW480 cells was induced by small interfering RNA (siRNA), and the cells were divided into three groups: untreated control, nonsense siRNA-treated control, and MALAT1 siRNA-treated group. SW480 cell apoptosis was assessed using TUNEL assay and flow cytometry. Apoptosis-related and Wnt/β-catenin signaling pathway-related proteins were detected by Western blotting in SW480 cells. SW480 cell proliferation was assessed by CCK-8 assay. MALAT1 expression was significantly higher in colon cancer vs. para-carcinoma tissues. Knockdown of MALAT1 by siRNA increased the number of apoptotic cells and the apoptosis rate at 24 h post-transfection in SW480 cells. Bcl2 associated X protein (Bax) expression was increased, B-cell lymphoma 2 (Bcl-2) expression was decreased, and the ratio of cleaved caspase-3 to truncated caspase-3 was increased in MALAT1 siRNA-transfected SW480 cells. MALAT1 knockdown decreased the proliferation of SW480 cells at 24 h, 48 h, and 72 h post-transfection. Wnt and β-catenin expression was inhibited in MALAT1 siRNA-transfected SW480 cells. Inhibition of MALAT1 expression in colon cancer may promote apoptosis and hinder cell proliferation by suppressing the activation of Wnt/β-catenin signaling pathway.
转移相关肺腺癌转录物 1（MALAT1）是一种高度保守的长非编码 RNA，与包括癌症在内的各种病理过程有关。MALAT1 在结肠癌中的作用和机制尚不清楚。我们研究了结肠癌组织中 MALAT1 的表达，MALAT1 对 SW480 细胞增殖和凋亡的影响，以及 MALAT1 作用涉及的信号通路。采用逆转录聚合酶链反应（RT-PCR）检测 60 例结肠癌及癌旁组织中 MALAT1 的表达。用小干扰 RNA（siRNA）诱导 SW480 细胞 MALAT1 敲低，将细胞分为三组：未处理对照、无意义 siRNA 处理对照和 MALAT1 siRNA 处理组。TUNEL 检测和流式细胞术评估 SW480 细胞凋亡。Western blot 检测 SW480 细胞中凋亡相关和 Wnt/β-catenin 信号通路相关蛋白。CCK-8 法检测 SW480 细胞增殖。结肠癌组织中 MALAT1 的表达明显高于癌旁组织。siRNA 敲低 MALAT1 可增加转染后 24 h SW480 细胞凋亡细胞数量和凋亡率。Bcl2 相关 X 蛋白（Bax）表达增加，B 细胞淋巴瘤 2（Bcl-2）表达减少，MALAT1 siRNA 转染的 SW480 细胞中裂解型 caspase-3 与截断型 caspase-3 的比值增加。MALAT1 敲低可降低转染后 24、48 和 72 h SW480 细胞的增殖。MALAT1 siRNA 转染的 SW480 细胞中 Wnt 和 β-catenin 的表达受到抑制。抑制结肠癌中 MALAT1 的表达可能通过抑制 Wnt/β-catenin 信号通路的激活来促进凋亡并阻碍细胞增殖。
