Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir, c/Guillem de Castro 94, Valencia 46001, Spain.
NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6-8, N-7491 Trondheim, Norway.
Int J Biol Macromol. 2020 Jan 15;143:1000-1008. doi: 10.1016/j.ijbiomac.2019.10.034. Epub 2019 Nov 15.
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a microbial biodegradable polymer with a wide range of potential industrial applications. However, its biomedical uses could increase exponentially if certain physical and biological properties were enhanced without compromising on the non-cytotoxic property of this biocompatible polymer. Graphene oxide (GO) nanosheets and carbon nanofibers (CNFs) have proven to be very promising reinforcing agents for the development of new composite materials. Therefore, PHBV films were prepared with 1% w/w of GO nanosheets or CNFs with the aim of enhancing their compression performance, thermal behaviour, wettability and cell adhesion using canine adipose-derived mesenchymal stem cells, and antibacterial activity against the model bacterium Staphylococcus aureus. The results of this study showed that both nanomaterials produced similar enhancements of the physical properties. However, PHBV/GO exhibited higher proliferative activity against time, cell adhesion and antibacterial activity than that of PHBV/CNFs. Nonetheless, both PHBV/GO and PHBV/CNFs composite films have shown considerable promise for biomedical applications.
聚（3-羟基丁酸酯-共-3-羟基戊酸酯）（PHBV）是一种具有广泛潜在工业应用的微生物可生物降解聚合物。然而，如果在不损害这种生物相容性聚合物的非细胞毒性特性的情况下，增强其某些物理和生物特性，其在生物医学方面的用途可能会呈指数级增长。氧化石墨烯（GO）纳米片和碳纤维纳米纤维（CNF）已被证明是开发新型复合材料的非常有前途的增强剂。因此，制备了含有 1% w/w GO 纳米片或 CNF 的 PHBV 薄膜，目的是提高其压缩性能、热行为、润湿性和犬脂肪间充质干细胞的粘附性，并对模型细菌金黄色葡萄球菌具有抗菌活性。这项研究的结果表明，这两种纳米材料都对物理性能产生了类似的增强作用。然而，PHBV/GO 表现出比 PHBV/CNFs 更高的增殖活性、细胞粘附性和抗菌活性。尽管如此，PHBV/GO 和 PHBV/CNFs 复合薄膜在生物医学应用方面都显示出了相当大的潜力。
