Department of Biochemistry and Biotechnology, Ghent University, Krijgslaan 281, 9000 Ghent, Belgium.
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir, c/Guillem de Castro 94, 46001 Valencia, Spain.
Int J Mol Sci. 2019 Jul 23;20(14):3603. doi: 10.3390/ijms20143603.
Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. Most recent studies have demonstrated the antibacterial activity and non-cytotoxicity of carbon nanomaterials such as graphene oxide (GO) and carbon nanofibers (CNFs). On the other hand, light-emitting diodes (LEDs) have shown great potential in a wide range of biomedical applications.
We investigated a nanotechnological strategy consisting of GO or CNFs combined with light-emitting diod (LED) irradiation as novel nanoweapons against two clinically relevant Gram-positive multidrug-resistant pathogens: methicillin-resistant (MRSA) and methicillin-resistant (MRSE). The cytotoxicity of GO and CNFs was studied in the presence of human keratinocyte HaCaT cells.
GO or CNFs exhibited no cytotoxicity and high antibacterial activity in direct contact with MRSE and MRSA cells. Furthermore, when GO or CNFs were illuminated with LED light, the MRSE and MRSA cells lost viability. The rate of decrease in colony forming units from 0 to 3 h, measured per mL, increased to 98.5 ± 1.6% and 95.8 ± 1.4% for GO and 99.5 ± 0.6% and 99.7 ± 0.2% for CNFs.
This combined antimicrobial approach opens up many biomedical research opportunities and provides an enhanced strategy for the prevention and treatment of Gram-positive multidrug-resistant infections.
由于目前全球范围内抗生素耐药性的出现，迫切需要寻找新的替代抗菌方法来应对多药耐药病原体。最近的研究表明，氧化石墨烯（GO）和碳纳米纤维（CNFs）等碳纳米材料具有抗菌活性和非细胞毒性。另一方面，发光二极管（LED）在广泛的生物医学应用中显示出巨大的潜力。
我们研究了一种纳米技术策略，该策略由 GO 或 CNFs 与发光二极管（LED）照射相结合，作为针对两种临床相关的革兰氏阳性多药耐药病原体的新型纳米武器：耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林表皮葡萄球菌（MRSE）。在存在人角质形成细胞 HaCaT 细胞的情况下研究了 GO 和 CNFs 的细胞毒性。
GO 或 CNFs 与 MRSE 和 MRSA 细胞直接接触时无细胞毒性且具有高抗菌活性。此外，当用 LED 光照射 GO 或 CNFs 时，MRSE 和 MRSA 细胞失去活力。每毫升测量的从 0 到 3 小时的集落形成单位减少率分别增加到 98.5 ± 1.6%和 95.8 ± 1.4%的 GO 和 99.5 ± 0.6%和 99.7 ± 0.2%的 CNFs。
这种联合抗菌方法为生物医学研究提供了许多机会，并为革兰氏阳性多药耐药感染的预防和治疗提供了增强的策略。
