Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore campus, Lahore, Pakistan.
Department of Pharmacology, Government College University Faisalabad, Faisalabad, Pakistan.
Inflammopharmacology. 2021 Apr;29(2):393-408. doi: 10.1007/s10787-020-00783-7. Epub 2021 Jan 2.
The current study was designed to evaluate the anti-oxidant and anti-arthritic potential of a traditionally used herb, Monotheca buxifolia. The M. buxifolia methanolic extract (MBME) was prepared from the aerial parts of the plant followed by chemical characterization with GC-MS. The anti-oxidant potential of the MBME was demonstrated by DPPH scavenging activity. The effects of MBME on protein denaturation and membrane stabilization were determined by inhibition of egg albumin denaturation and RBC membrane stabilization assays, respectively. The in vivo anti-arthritic potential of the MBME at 50, 100, and 150 mg/kg/day was evaluated in Complete Freund's Adjuvant-induced polyarthritis in Wistar rats treated for 21 days. Phytochemicals, such as linolenic acid methyl ester, n-hexadecanoic acid, vitamin E, α-amyrin, and β-amyrin were detected in the GC-MS analysis. The plant extract exhibited a 55.20 ± 0.69% scavenging of free radicals at 100 µg/ml concentration. It significantly (p < 0.05) stabilized human RBC membrane (65.06 ± 0.22%) and inhibited protein denaturation (70.53 ± 0.34%) at 100 mg/ml concentration. The diclofenac sodium (DS) and MBME at 150,100, and 50 mg/kg reduced the paw edema, restored the body weight, and altered blood parameters including CRP. The MBME significantly reduced the MDA and increased the SOD, CAT, and GSH levels in liver tissue homogenate in treated rats. The serum concentration of TNF-α and PGE2 was remarkably (p < 0.01-< 0.0001) restored by the DS and MBME dose dependently. The histopathological study showed that MBME 150 mg/kg commendably restored the ankle joint inflammation, bone erosion, and cartilage damage in polyarthritic rats. It was concluded that the anti-oxidant, anti-inflammatory and anti-arthritic effects of MBME might be attributed to phenols, flavonoids, triterpenoids, vitamin E, phytol, and other fatty acids. This study showed the anti-arthritic potential of Monotheca buxifolia and thus validates its traditional claim.
本研究旨在评估传统药用植物 Monotheca buxifolia 的抗氧化和抗关节炎潜力。从该植物的地上部分提取 Monotheca buxifolia 甲醇提取物（MBME），并通过 GC-MS 进行化学表征。通过 DPPH 清除活性评价 MBME 的抗氧化潜力。通过抑制卵清蛋白变性和 RBC 膜稳定性测定，分别测定 MBME 对蛋白质变性和膜稳定性的影响。MBME 在 50、100 和 150 mg/kg/天的剂量下，在完全弗氏佐剂诱导的 Wistar 大鼠多关节炎模型中进行了 21 天的体内抗关节炎评价。在 GC-MS 分析中检测到亚麻酸甲酯、正十六烷酸、维生素 E、α-熊果苷和 β-熊果苷等植物化学物质。该植物提取物在 100 µg/ml 浓度下对自由基的清除率为 55.20 ± 0.69%。它在 100 mg/ml 浓度下显著（p < 0.05）稳定人 RBC 膜（65.06 ± 0.22%）并抑制蛋白质变性（70.53 ± 0.34%）。双氯芬酸钠（DS）和 MBME 在 150、100 和 50 mg/kg 剂量下可减少爪肿胀，恢复体重，并改变包括 CRP 在内的血液参数。MBME 可显著降低 MDA 水平，并增加肝组织匀浆中的 SOD、CAT 和 GSH 水平。DS 和 MBME 剂量依赖性地显著（p < 0.01-< 0.0001）恢复了 TNF-α 和 PGE2 的血清浓度。组织病理学研究表明，MBME 150 mg/kg 可显著改善多关节炎大鼠踝关节炎症、骨侵蚀和软骨损伤。结论：MBME 的抗氧化、抗炎和抗关节炎作用可能归因于酚类、类黄酮、三萜类、维生素 E、植醇和其他脂肪酸。本研究显示了 Monotheca buxifolia 的抗关节炎潜力，从而验证了其传统功效。
