Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, 646000, China.
Chengdu Vocational and Technical College, Chengdu, 610000, China.
Curr Med Sci. 2022 Aug;42(4):711-719. doi: 10.1007/s11596-022-2594-4. Epub 2022 Aug 13.
The present study explored the mechanisms involved in intestinal lymphatic ligation in rats with severe acute pancreatitis (SAP).
Male Sprague Dawley rats were randomly divided into 4 groups: saline group, saline+ligation group, SAP group, and SAP+ligation group. Rats in the SAP group were administered sodium taurocholate solution. Isolated mesenteric lymph duct ligation was administered to the saline+ligation and SAP+ligation groups. Endotoxin (ET), nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), myeloperoxidase (MPO), and superoxide dismutase (SOD) were detected. Nuclear factor-κB (NF-κB) and intercellular cell adhesion molecule-1 (ICAM-1) proteins were observed. The mRNA of inducible nitric oxide synthase(iNOS) and Toll-like receptor 4 (TLR4) was detected by PCR.
Pathomorphological analysis showed that necrosis was present in the lung of rats in the SAP group, but only mild lesions in the SAP+ligation group. ET, NO, TNF-α, and IL-1 in the serum and lung tissue were significantly decreased and MPO was increased in the SAP+ligation group as compared with the SAP group. However, MPO was increased. The expression of NF-κB and ICAM-1, either iNOS or TLR4, was upregulated in the SAP group, but downregulated in the SAP+ligation group. Intestinal lymph duct ligation prevented ET translocation, the release of inflammation factors, and inflammation injury.
The intestinal lymph duct ligation could alleviate SAP-induced pulmonary injury by suppressing NF-κB activation in rats.
本研究旨在探讨严重急性胰腺炎（SAP）大鼠肠淋巴管结扎相关的机制。
雄性 Sprague Dawley 大鼠随机分为 4 组：生理盐水组、生理盐水+结扎组、SAP 组和 SAP+结扎组。SAP 组大鼠给予牛磺胆酸钠溶液。结扎分离肠系膜淋巴管，应用于生理盐水+结扎组和 SAP+结扎组。检测内毒素（ET）、一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）、髓过氧化物酶（MPO）和超氧化物歧化酶（SOD）。观察核因子-κB（NF-κB）和细胞间黏附分子-1（ICAM-1）蛋白。通过 PCR 检测诱导型一氧化氮合酶（iNOS）和 Toll 样受体 4（TLR4）的 mRNA。
形态学分析表明，SAP 组大鼠肺组织出现坏死，但 SAP+结扎组仅出现轻度病变。与 SAP 组相比，SAP+结扎组血清和肺组织中 ET、NO、TNF-α 和 IL-1 明显减少，MPO 增加。但 MPO 增加。SAP 组 NF-κB 和 ICAM-1 表达上调，iNOS 或 TLR4 表达上调，但 SAP+结扎组 NF-κB 和 ICAM-1 表达下调，iNOS 或 TLR4 表达下调。肠淋巴管结扎可防止 ET 易位、炎症因子释放和炎症损伤。
在大鼠中，肠淋巴管结扎可通过抑制 NF-κB 激活来减轻 SAP 诱导的肺损伤。
