Molecular and Cell Biology, Saarland University, Saarbrücken, Germany.
Department of Pharmacy, Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbrücken, Germany.
Biotechnol Bioeng. 2020 Mar;117(3):776-788. doi: 10.1002/bit.27226. Epub 2019 Nov 28.
Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering the body. Therefore, we investigated the interaction of biodegradable surface-modified poly(lactic-co-glycolic acid) (PLGA) particles with yeast cells to overcome the unspecificity of the particulate carriers. Cells of different Saccharomyces cerevisiae strains were characterized regarding their interaction with PLGA-NPs under isotonic and hypotonic conditions. The particles were shown to efficiently interact with yeast cells leading to stable NP/yeast-complexes allowing to associate or even internalize compounds. Notably, applying those complexes to a coculture model of HeLa cells and macrophages, the macrophages were specifically targeted. This novel nano-in-micro carrier system suggests itself as a promising tool for the delivery of biologically active agents into phagocytic cells combining specificity and efficiency.
纳米颗粒（NPs）能够将各种物质递送到真核细胞中。然而，由于缺乏特异性，它们的使用常常受到阻碍，导致几乎所有细胞类型都不希望摄取 NPs。相比之下，酵母被已知在进入体内后会被专门摄取到免疫细胞中。因此，我们研究了可生物降解的表面修饰的聚（乳酸-共-羟基乙酸）（PLGA）颗粒与酵母细胞的相互作用，以克服颗粒载体的非特异性。在等渗和低渗条件下，对不同的酿酒酵母菌株的细胞与 PLGA-NPs 的相互作用进行了表征。结果表明，这些颗粒能够有效地与酵母细胞相互作用，形成稳定的 NP/酵母复合物，从而能够结合甚至内化化合物。值得注意的是，将这些复合物应用于 HeLa 细胞和巨噬细胞的共培养模型中，巨噬细胞被特异性靶向。这种新型纳米微载体系统本身可作为将生物活性物质递送到吞噬细胞中的有前途的工具，结合了特异性和效率。
