Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
Biotechnol Bioeng. 2020 Mar;117(3):832-843. doi: 10.1002/bit.27228. Epub 2019 Nov 28.
The creation of a blueprint for stem cell bioprocess development that it is easily readable and shareable among those involved in the construction of the bioprocess is a necessary step toward full-fledged bioprocess integration. The blueprint provides the culturing tools and methodologies, designed to highlight knowledge gaps within biological sciences and bioengineering. This review highlights a blueprint for stem cell bioprocessing development using a landscape architecture approach that can aid the development of culture technologies and tools that satisfy the demands for stem cell-derived products for use in clinical and industrial applications. This work is intended to provide insights to cell biologists, geneticists, bioengineers, and clinicians seeking knowledge outside of their field of expertise and fosters a leap from a reductionist approach to one, that is, globally integrated in stem cell bioprocessing.
创建一个易于阅读且可在参与生物工艺构建的人员之间共享的干细胞生物工艺开发蓝图，是实现全面生物工艺整合的必要步骤。该蓝图提供了培养工具和方法，旨在突出生物科学和生物工程中的知识空白。本综述强调了使用景观建筑学方法开发干细胞生物工艺的蓝图，该方法可以帮助开发满足临床和工业应用中干细胞衍生产品需求的培养技术和工具。这项工作旨在为寻求专业领域以外知识的细胞生物学家、遗传学家、生物工程师和临床医生提供见解，并促进从还原论方法向全面集成的干细胞生物工艺方法的飞跃。
