Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA, USA.
Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA, USA.
Trends Biotechnol. 2020 Oct;38(10):1128-1140. doi: 10.1016/j.tibtech.2020.03.006. Epub 2020 Apr 5.
Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods that measure and monitor intrinsic markers of cell differentiation state, developmental status, and viability in real time. In addition, proper cell culture modalities that nurture the growth of high-quality stem cells in suspension are critical for industrial scale-up. In this review, we present an overview of cell culture media, suspension modalities, and monitoring techniques that preserve the quality and pluripotency of iPSCs during initiation, expansion, and manufacturing.
大规模生产诱导多能干细胞（iPSCs）对于治疗多种临床适应症至关重要。然而，由于 iPSCs 具有敏感的多能状态和对支持基质的依赖性，培养足够数量的 iPSCs 用于临床应用是有问题的。开发可扩展且满足临床需求的干细胞生物加工策略需要结合实时测量和监测细胞分化状态、发育状态和活力内在标志物的方法。此外，适当的细胞培养方式对于在悬浮状态下培养高质量干细胞的生长至关重要。在这篇综述中，我们介绍了细胞培养基、悬浮方式和监测技术，这些技术在 iPSCs 的起始、扩增和制造过程中保持了 iPSCs 的质量和多能性。
