盐酸青藤碱在应激负荷诱导的心力衰竭中的保护作用及其机制。
Sinomenine's protective role and mechanism in stress load-induced heart failure.
机构信息
Grade 10 Pharmacy Undergraduate, School of Pharmacy, Zhejiang University of Technology, Hangzhou, China.
School of Pharmacy, Zhejiang University of Technology, Hangzhou, China.
出版信息
J Pharm Pharmacol. 2020 Feb;72(2):209-217. doi: 10.1111/jphp.13181. Epub 2019 Nov 17.
OBJECTIVES
This study is designed to investigate the effects and mechanisms of sinomenine (Sin) in stress load-induced heart failure in mice.
METHODS
We used aortic constriction (AB) to cause pressure overload as our heart failure model. Sin was received in mice as the treatment group. Cardiac function and structural changes were detected using echocardiography. Heart-lung mass ratios were measured. The serum levels of IL-10 and IL-17 proteins were detected by using ELISA, cardiac hypertrophy markers atrial natriuretic peptide (ANP), myocardial I and III collagen mRNA levels were detected by RT-PCR. Myocardial type I and III collagen protein levels were detected by Western blotting.
KEY FINDINGS
Sin significantly improved stress load-induced heart failure (P < 0.05), reduced the heart-lung mass ratio, ANP, collagen-I and -III mRNA and protein levels (P < 0.05); Sin can enhance the ratio of IL-10/IL-17.
CONCLUSION
Sin may be a promising drug target to improve heart failure. Its role is related to reduce serum ANP levels, inhibit the mRNA and protein level of type I and III collagen and enhance the ratio of IL-10/IL-17.
目的
本研究旨在探讨青藤碱（Sin）在压力负荷诱导的小鼠心力衰竭中的作用及其机制。
方法
采用主动脉缩窄（AB）造成压力超负荷作为心力衰竭模型，用青藤碱（Sin）处理小鼠作为治疗组。通过超声心动图检测心功能和结构变化，测量心肺重量比。采用 ELISA 法检测血清白细胞介素 10（IL-10）和白细胞介素 17（IL-17）蛋白水平，采用 RT-PCR 法检测心房利钠肽（ANP）、心肌 I 和 III 型胶原 mRNA 水平，采用 Western blot 法检测心肌 I 和 III 型胶原蛋白水平。
主要发现
青藤碱显著改善压力负荷诱导的心力衰竭（P<0.05），降低心肺重量比、ANP、胶原-I 和 -III mRNA 和蛋白水平（P<0.05）；青藤碱能提高 IL-10/IL-17 比值。
结论
青藤碱可能是改善心力衰竭的有前途的药物靶点。其作用与降低血清 ANP 水平、抑制 I 型和 III 型胶原的 mRNA 和蛋白水平以及提高 IL-10/IL-17 比值有关。
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