Wiinholt Alexander, Jørgensen Mads G, Bučan Amar, Dalaei Farima, Sørensen Jens A
University of Southern Denmark; Department of Plastic Surgery, Odense University Hospital.
Department of Plastic Surgery, Odense University Hospital.
J Vis Exp. 2019 Nov 2(153). doi: 10.3791/60578.
Animal models are of paramount importance in the research of lymphedema in order to understand the pathophysiology of the disease but also to explore potential treatment options. This mouse model allows researchers to induce significant lymphedema lasting at least 8 weeks. Lymphedema is induced using a combination of fractioned radiotherapy and surgical ablation of lymphatics. This model requires that the mice get a dose of 10 Gray (Gy) radiation before and after surgery. The surgical part of the model involves ligation of three lymph vessels and extraction of two lymph nodes from the mouse hindlimb. Having access to microsurgical tools and a microscope is essential, due to the small anatomical structures of mice. The advantage of this model is that it results in statistically significant lymphedema, which provides a good basis for evaluating different treatment options. It is also a great and easily available option for microsurgical training. The limitation of this model is that the procedure can be time consuming, especially if not practiced in advance. The model results in objectively quantifiable lymphedema in mice, without causing severe morbidity and has been tested in three separate projects.
动物模型在淋巴水肿研究中至关重要，这不仅有助于理解该疾病的病理生理学，还能探索潜在的治疗方案。这种小鼠模型使研究人员能够诱导出至少持续8周的显著淋巴水肿。淋巴水肿通过分割放疗和淋巴管手术切除相结合的方法诱导产生。该模型要求小鼠在手术前后接受10格雷（Gy）的辐射剂量。模型的手术部分包括结扎三条淋巴管并从小鼠后肢提取两个淋巴结。由于小鼠解剖结构较小，因此使用显微手术工具和显微镜至关重要。该模型的优点是能产生具有统计学意义的淋巴水肿，为评估不同治疗方案提供了良好基础。它也是显微手术训练的一个很好且易于获得的选择。该模型的局限性在于该操作可能耗时，尤其是如果事先没有练习过。该模型在小鼠中产生客观上可量化的淋巴水肿，不会导致严重发病，并且已经在三个独立项目中进行了测试。
