Samova Sanman, Doctor Hetal, Verma Ramtej
Department of Zoology B.M.T.C and Human Genetic, School of Sciences, Gujarat University, Ahmedabad, Gujarat, India.
Interdiscip Toxicol. 2018 Oct;11(3):209-216. doi: 10.2478/intox-2018-0019. Epub 2019 Oct 18.
Bisphenol A is widely used as a material for the production of epoxy resins and polycarbonate plastics. It contaminates various food stuffs by getting leached out from their container lining. Limited information is available on its effects on the male reproductive system. The aim of the present study was to evaluate the extent to which bisphenol A can affect the reproductive system by measuring biochemical and histological changes in the epididymis. Inbred Swiss strain male albino mice were orally administered 80, 120 and 240 mg/kg body weight/day of BPA for 45 days. After completion of treatment, the animals were sacrificed; cauda epididymis was isolated, weighed, used for biochemical and histopathological studies. The results revealed that BPA administered for 45 days caused significant (0.05) and dose-dependent reduction in epididymis weight. There was significant (0.05) increase in lipid peroxidation and the acid phosphatase activity. Dose dependent reduction in protein, sialic acid contents, as well as the activity of enzymatic antioxidants and mitochondrial enzymes was recorded compared to vehicle treated group. The effect was dose-dependent. Histopathological alteration was observed. This study concludes that BPA causes toxicity in epididymis of mice by generating free radicals, which may be a possible reason for reduction in sperm parameters.
双酚A被广泛用作生产环氧树脂和聚碳酸酯塑料的材料。它会从食品容器衬里中渗出，从而污染各种食品。关于其对雄性生殖系统影响的信息有限。本研究的目的是通过测量附睾的生化和组织学变化来评估双酚A对生殖系统的影响程度。将近交系瑞士品系雄性白化小鼠每天口服80、120和240毫克/千克体重的双酚A，持续45天。治疗结束后，处死动物；分离附睾尾部，称重，用于生化和组织病理学研究。结果显示，连续45天给予双酚A导致附睾重量显著（P<0.05）且呈剂量依赖性降低。脂质过氧化和酸性磷酸酶活性显著（P<0.05）增加。与溶剂处理组相比，蛋白质、唾液酸含量以及酶促抗氧化剂和线粒体酶的活性呈剂量依赖性降低。这种影响是剂量依赖性的。观察到了组织病理学改变。本研究得出结论，双酚A通过产生自由基对小鼠附睾造成毒性，这可能是精子参数降低的一个可能原因。
