Hyun JongYoung, Do Hoang Dang Khoa, Jung Joonhyung, Kim Joo-Hwan
Department of Life Science, Gachon University, Seongnam, Gyeonggi, Korea.
PeerJ. 2019 Nov 11;7:e7965. doi: 10.7717/peerj.7965. eCollection 2019.
Genomic data play an important role in plant research because of its implications in studying genomic evolution, phylogeny, and developing molecular markers. Although the information of invasive alien plants was collected, the genomic data of those species have not been intensively studied.
We employ the next generation sequencing and PCR methods to explore the genomic data as well as to develop and test the molecular markers.
In this study, we characterize the chloroplast genomes (cpDNA) of and , of which the lengths are 137,144 and 137,131 bp, respectively. These two newly sequenced genomes include 78 protein-coding genes, 30 tRNA, and four rRNA. There are 56 simple single repeats and 17 forward repeats in the chloroplast genome of . Most of the repeats locate in non-coding regions. However, repeats can be found in , and . The phylogenomic analysis revealed the monophyly of but not species in tribe Paniceae. The single nucleotide polymorphism sites in , and were successfully used for developing molecular markers to distinguish and related taxa. The simple PCR protocol for using the newly developed molecular markers was also provided.
基因组数据在植物研究中发挥着重要作用，因为其对研究基因组进化、系统发育以及开发分子标记具有重要意义。尽管已收集了外来入侵植物的信息，但尚未对这些物种的基因组数据进行深入研究。
我们采用新一代测序和PCR方法来探索基因组数据，并开发和测试分子标记。
在本研究中，我们对[物种1]和[物种2]的叶绿体基因组（cpDNA）进行了表征，其长度分别为137,144和137,131 bp。这两个新测序的基因组包含78个蛋白质编码基因、30个tRNA和4个rRNA。[物种1]的叶绿体基因组中有56个简单单重复序列和17个正向重复序列。大多数重复序列位于非编码区。然而，在[物种2]中也能发现重复序列。系统发育基因组分析揭示了黍族中[物种1]的单系性，但未揭示[物种2]的单系性。[物种1]、[物种2]和[物种3]中的单核苷酸多态性位点成功用于开发分子标记以区分[物种1]及其相关类群。还提供了使用新开发分子标记的简单PCR方案。
