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开发一种基于单核苷酸多态性（SNP）的高通量标记系统，以促进性状从物种渗入到面包小麦中（）。

Developing a High-Throughput SNP-Based Marker System to Facilitate the Introgression of Traits From Species Into Bread Wheat ().

作者信息

Przewieslik-Allen Alexandra M, Burridge Amanda J, Wilkinson Paul A, Winfield Mark O, Shaw Daniel S, McAusland Lorna, King Julie, King Ian P, Edwards Keith J, Barker Gary L A

机构信息

Life Sciences, University of Bristol, Bristol, United Kingdom.

Plant Sciences, Sutton Bonington Campus, Leicestershire, United Kingdom.

出版信息

Front Plant Sci. 2019 Jan 24;9:1993. doi: 10.3389/fpls.2018.01993. eCollection 2018.

DOI:10.3389/fpls.2018.01993
PMID:30733728
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6354564/
Abstract

The genus contains a diverse collection of wild species exhibiting variation in geographical distribution, ecological adaptation, ploidy and genome organization. is the most closely related genus to which includes cultivated wheat, a globally important crop that has a limited gene pool for modern breeding. species are a potential future resource for wheat breeding for traits, such as adaptation to different ecological conditions and pest and disease resistance. This study describes the development and application of the first high-throughput genotyping platform specifically designed for screening wheat relative species. The platform was used to screen multiple accessions representing all species in the genus . Firstly, the data was demonstrated to be useful for screening diversity and examining relationships within and between species. Secondly, markers able to characterize and track introgressions from species in hexaploid wheat were identified and validated using two different approaches.

摘要

该属包含多种野生种，这些野生种在地理分布、生态适应、倍性和基因组组织方面表现出差异。它是与包含栽培小麦的属关系最密切的属，栽培小麦是一种全球重要作物，但其现代育种的基因库有限。该属物种是未来小麦育种中获取如适应不同生态条件以及抗病虫害等性状的潜在资源。本研究描述了首个专门设计用于筛选小麦近缘物种的高通量基因分型平台的开发与应用。该平台用于筛选代表该属所有物种的多个种质。首先，数据被证明可用于筛选该属物种内和物种间的多样性并检验它们之间的关系。其次，使用两种不同方法鉴定并验证了能够表征和追踪六倍体小麦中该属物种渗入情况的标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/ab8af4c4b360/fpls-09-01993-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/eb5d51693620/fpls-09-01993-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/2b327770bfa7/fpls-09-01993-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/f6ba887db1cc/fpls-09-01993-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/5ca945b4c269/fpls-09-01993-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/dda85b6d0ff0/fpls-09-01993-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/ab8af4c4b360/fpls-09-01993-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/eb5d51693620/fpls-09-01993-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/2b327770bfa7/fpls-09-01993-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/f6ba887db1cc/fpls-09-01993-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/5ca945b4c269/fpls-09-01993-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/dda85b6d0ff0/fpls-09-01993-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6fd/6354564/ab8af4c4b360/fpls-09-01993-g0006.jpg

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Genome Targeted Introgression of Resistance to African Stem Rust from into Bread Wheat.
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