用 NaOH/尿素溶液冻融预处理法从三疣梭子蟹（Portunus trituberculatus）壳中制备壳聚糖并对其进行表征。
Preparation and characterization of chitosan from crab shell (Portunus trituberculatus) by NaOH/urea solution freeze-thaw pretreatment procedure.
机构信息
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China; Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, PR China.
出版信息
Int J Biol Macromol. 2020 Mar 15;147:931-936. doi: 10.1016/j.ijbiomac.2019.10.059. Epub 2019 Nov 15.
The effect of NaOH/urea solution freeze-thaw pretreatment to the chitosan of purity and the degree of deacetylation from the crab shell was examined. Higher purity chitosan (CS1) was prepared. On this basis, the raw materials after the first demineralization were pretreated with NaOH/urea solution freeze-thaw under different freeze-thaw situations. The produced chitosans (CS1-CS4) were characterized in the ash content, solubility, protein content, degree of deacetylation, viscosity average molecular weight, SEM, FTIR, XRD, antimicrobial activity. The results showed that the freeze-thaw process had advantageous influence on decreasing the ash content and increasing degree of deacetylation and antibacterial activity. Moreover, the antibacterial property of the extracted chitosans seemed to be positively related to their degree of deacetylation. It is concluded that CS4 had the lowest ash content (0.052%), the highest degree of deacetylation (86.02%) and the greatest antibacterial activity. Therefore, we recommend CS4 as agent for industrial and pharmaceutical applications.
考察了 NaOH/尿素溶液冻融预处理对蟹壳来源壳聚糖的纯度和脱乙酰度的影响。制备了更高纯度的壳聚糖（CS1）。在此基础上，用 NaOH/尿素溶液对第一次脱矿后的原料在不同的冻融条件下进行冻融预处理。对所制备的壳聚糖（CS1-CS4）的灰分含量、溶解度、蛋白质含量、脱乙酰度、黏均分子量、SEM、FTIR、XRD 和抗菌活性进行了表征。结果表明，冻融过程有利于降低灰分含量、提高脱乙酰度和抗菌活性。此外，提取的壳聚糖的抗菌性能似乎与其脱乙酰度呈正相关。结论是 CS4 具有最低的灰分含量（0.052%）、最高的脱乙酰度（86.02%）和最大的抗菌活性。因此，我们推荐 CS4 作为工业和医药应用的试剂。
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