Li Yunfeng, Zhai Lihong, Fan Jingsheng, Ren Jiaxin, Gong Wenrong, Wang Xin, Huang Jun
Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Genetica. 2019 Dec;147(5-6):391-400. doi: 10.1007/s10709-019-00080-4. Epub 2019 Nov 18.
HECT (homologous to the E6AP carboxyl terminus) ubiquitin ligase genes (E3s) are enzymes with diverse functions influencing plant growth, development, and responses to abiotic stresses. However, there is relatively little information available regarding the maize HECT E3 gene family. In the present study, 12 maize HECT E3 genes (ZmUPL1 to ZmUPL12) were identified at the whole-genome level. The phylogenetic relationships, structures, and expression levels of the maize HECT E3 genes were then analyzed. On the basis of the constructed maximum likelihood phylogenetic tree, the HECT E3 genes were divided into six groups. The quantitative real-time polymerase chain reaction assay results revealed that all of the maize ZmUPL genes were expressed in most of the examined tissues and were responsive to three abiotic stresses. Considered together, the study results may provide a useful foundation for future investigations of maize stress-tolerance genes as well as functional analyses of the E3 enzymes in diverse agriculturally important crop species.
HECT（与E6AP羧基末端同源）泛素连接酶基因（E3s）是一类具有多种功能的酶，影响植物生长、发育及对非生物胁迫的响应。然而，关于玉米HECT E3基因家族的信息相对较少。在本研究中，在全基因组水平上鉴定出12个玉米HECT E3基因（ZmUPL1至ZmUPL12）。随后分析了玉米HECT E3基因的系统发育关系、结构和表达水平。基于构建的最大似然系统发育树，HECT E3基因被分为六组。定量实时聚合酶链反应分析结果表明，所有玉米ZmUPL基因在大多数检测组织中均有表达，且对三种非生物胁迫有响应。综合来看，研究结果可能为未来玉米耐逆基因的研究以及多种重要农作物中E3酶的功能分析提供有用的基础。
