Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2022 Sep;43:101012. doi: 10.1016/j.cbd.2022.101012. Epub 2022 Jul 5.
E3 ubiquitin ligase (E3s) plays an important role in ubiquitin proteasome pathway, proteins containing homologous E6-AP carboxyl terminus (HECT) domains. However, the role of HECT E3 ubiquitin ligase in mollusk was rarely explored. In this study, we performed a genome-wide analysis of the HECT domain-containing gene in Ruditapes philippinarum to identify and predict the structural and functional characterization of HECT genes in response to abiotic and biotic stress. A total of sixteen members of HECT gene family were identified and analyzed for the gene structure, phylogenetic relation, three-dimensional structure, protein interaction network, and expression patterns. Experimental results demonstrated that Rph.HUWE1, Rph.HECTD1, Rph.Ubr5 were significantly up-regulated in response to heat stress and bacterial challenge. Taken together, our data provide insights into the potential function of HECT E3 ligase in heat stress and Vibrio anguillarum infection.
E3 泛素连接酶（E3s）在泛素蛋白酶体途径中发挥着重要作用，该途径包含同源 E6-AP 羧基末端（HECT）结构域的蛋白质。然而，HECT E3 泛素连接酶在软体动物中的作用很少被探索。在这项研究中，我们对菲律宾蛤仔中的 HECT 结构域基因进行了全基因组分析，以鉴定和预测 HECT 基因在应对非生物和生物胁迫时的结构和功能特征。总共鉴定和分析了十六个 HECT 基因家族成员的基因结构、系统发育关系、三维结构、蛋白质相互作用网络和表达模式。实验结果表明，Rph.HUWE1、Rph.HECTD1 和 Rph.Ubr5 在热应激和细菌刺激下的表达显著上调。总之，我们的数据为 HECT E3 连接酶在热应激和鳗弧菌感染中的潜在功能提供了新的见解。
