Department of Emergency and Organ Transplantation, Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases, University of Bari Aldo Moro, Bari, Italy.
Diabetes Metab Res Rev. 2020 Mar;36(3):e3238. doi: 10.1002/dmrr.3238. Epub 2019 Dec 19.
Irisin, a newly discovered muscle-derived hormone, acts in different organs and tissues, improving energy homeostasis. In this study, we assessed, for the first time, the effects of intraperitoneal irisin injections on circulating levels of leptin and ghrelin, mRNA expression of the major hypothalamic appetite regulators and brain neurotrophic factors, as well as feeding behaviour in healthy mice.
Twelve male 6-week-old C57BL/6 mice were randomized into two groups and intraperitoneally injected daily with irisin (0.5 μg/g body weight) or vehicle (phosphate-buffered saline [PBS]) for 14 days. On the last day of observation, leptin and ghrelin levels were measured with an enzyme-linked immunosorbent assay (ELISA). mRNA levels of genes of interest were analysed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in brain extracts.
Irisin administration did not change leptin or ghrelin serum concentrations. However, irisin injection increased CART, POMC, NPY, and BDNF mRNA levels, without affecting the mRNA expression of AgRP, orexin, PMCH, and UCP2. Finally, over the time frame of irisin treatment, body weight and feeding behaviour were unaltered.
These results suggest that intraperitoneal injection of irisin, although without effects on feeding behaviour and body weight, can increase the expression of anorexigenic and neurotrophic genes in mouse brain.
鸢尾素是一种新发现的肌肉源性激素，在不同的器官和组织中发挥作用，改善能量稳态。本研究首次评估了腹腔内注射鸢尾素对健康小鼠循环瘦素和胃饥饿素水平、主要下丘脑食欲调节因子和脑神经营养因子 mRNA 表达以及摄食行为的影响。
将 12 只 6 周龄雄性 C57BL/6 小鼠随机分为两组，每日腹腔内注射鸢尾素（0.5μg/g 体重）或载体（磷酸盐缓冲盐水[PBS]），共 14 天。在观察的最后一天，用酶联免疫吸附试验（ELISA）测量瘦素和胃饥饿素水平。用定量逆转录聚合酶链反应（qRT-PCR）分析脑提取物中感兴趣基因的 mRNA 水平。
鸢尾素给药并未改变瘦素或胃饥饿素的血清浓度。然而，鸢尾素注射增加了 CART、POMC、NPY 和 BDNF 的 mRNA 水平，而不影响 AgRP、orexin、PMCH 和 UCP2 的 mRNA 表达。最后，在鸢尾素治疗的时间范围内，体重和摄食行为没有改变。
这些结果表明，尽管腹腔内注射鸢尾素对摄食行为和体重没有影响，但可以增加小鼠大脑中厌食和神经营养基因的表达。
