Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran,
Gynecol Obstet Invest. 2020;85(1):94-99. doi: 10.1159/000502820. Epub 2019 Nov 19.
The objective of the present study was to investigate the development of mouse embryos and the chromosomal status after the pre-implantation treatment with paclitaxel (Taxol) based on the reports that indicate Taxol improves the developmental potential of vitrified human and mouse oocytes.
Outbred female mice were superovulated and in vitro fertilization (IVF) was carried out using sperms from the same strain. Two-cell stage mouse embryos were cultured in the presence of Taxol for 24 h. After the determination of a non-toxic dose of Taxol, embryo development in control and Taxol-treated groups was compared during 3.5 days post-IVF. The aneuploidy rate of embryos was assessed by fluorescence in situ hybridization for chromosomes 2 and 11.
Development to morula and blastocyst stages was considerably enhanced following the addition of Taxol 0.01 µM compared to a similar situation in controls (p < 0.0001). Moreover, the degeneration rate was decreased following treatment with this concentration of Taxol (p < 0.01). The rate of aneuploidy in embryos and individual blastomeres did not vary between groups (p = 0.518 and 0.810 respectively).
Pre-implantation treatment with Taxol 0.01 µM had a positive effect on the development to morula/blastocyst stages and decreased the degeneration rate without affecting the aneuploidy rate of chromosomes 2 and 11.
本研究旨在探讨紫杉醇（Taxol）预处理对小鼠胚胎发育和染色体状态的影响，因为有报道称 Taxol 可提高玻璃化冷冻人卵和小鼠卵的发育潜能。
超排卵获得雌性外种系小鼠，用同一品系的精子进行体外受精（IVF）。将 2 细胞期的小鼠胚胎在 Taxol 存在的情况下培养 24 小时。确定 Taxol 的非毒性剂量后，比较对照组和 Taxol 处理组在 IVF 后 3.5 天的胚胎发育情况。通过荧光原位杂交技术检测染色体 2 和 11 的非整倍体率。
与对照组相比，添加 0.01µM Taxol 后，胚胎发育到桑葚胚和囊胚阶段的情况明显改善（p<0.0001）。此外，用该浓度的 Taxol 处理后，退化率降低（p<0.01）。胚胎和单个卵裂球的非整倍体率在各组之间没有差异（分别为 p=0.518 和 0.810）。
在桑葚胚/囊胚阶段，用 0.01µM Taxol 预处理对胚胎发育有积极影响，并降低了退化率，而不影响染色体 2 和 11 的非整倍体率。
