Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
School of Computer and Information Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Viruses. 2019 Nov 16;11(11):1070. doi: 10.3390/v11111070.
Chicken gout resulting from nephropathogenic infectious bronchitis virus (NIBV) has become a serious kidney disease problem in chicken worldwide with alterations of the metabolic phenotypes in multiple metabolic pathways. To investigate the mechanisms in chicken responding to NIBV infection, we examined the global transcriptomic and metabolomic profiles of the chicken's kidney using RNA-seq and GC-TOF/MS, respectively. Furthermore, we analyzed the alterations in cecal microorganism composition in chickens using 16S rRNA-seq. Integrated analysis of these three phenotypic datasets further managed to create correlations between the altered kidney transcriptomes and metabolome, and between kidney metabolome and gut microbiome. We found that 2868 genes and 160 metabolites were deferentially expressed or accumulated in the kidney during NIBV infection processes. These genes and metabolites were linked to NIBV-infection related processes, including immune response, signal transduction, peroxisome, purine, and amino acid metabolism. In addition, the comprehensive correlations between the kidney metabolome and cecal microbial community showed contributions of gut microbiota in the progression of NIBV-infection. Taken together, our research comprehensively describes the host responses during NIBV infection and provides new clues for further dissection of specific gene functions, metabolite affections, and the role of gut microbiota during chicken gout.
由肾致病性传染性支气管炎病毒（NIBV）引起的鸡痛风已成为全球鸡群中一种严重的肾脏疾病问题，其在多种代谢途径中的代谢表型发生改变。为了研究鸡对 NIBV 感染的反应机制，我们分别使用 RNA-seq 和 GC-TOF/MS 检测了鸡肾脏的全局转录组和代谢组学图谱。此外，我们使用 16S rRNA-seq 分析了鸡盲肠微生物组成的变化。这三个表型数据集的综合分析进一步管理创建了肾脏转录组和代谢组之间、肾脏代谢组和肠道微生物组之间的相关性。我们发现，在 NIBV 感染过程中，2868 个基因和 160 种代谢物在肾脏中差异表达或积累。这些基因和代谢物与 NIBV 感染相关过程有关，包括免疫反应、信号转导、过氧化物酶体、嘌呤和氨基酸代谢。此外，肾脏代谢组和盲肠微生物群落之间的综合相关性表明，肠道微生物群在 NIBV 感染进展中的作用。总之，我们的研究全面描述了 NIBV 感染期间宿主的反应，并为进一步剖析特定基因功能、代谢物影响以及肠道微生物群在鸡痛风中的作用提供了新的线索。
