载铁(II)的双光子激发荧光二氧化硅纳米粒子作为探针用于活细胞中过氧化氢的测定和成像。
Two-photon excited fluorescent silica nanoparticles loaded with iron(II) as a probe for determination and imaging of hydrogen peroxide in living cells.
机构信息
Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan, 453007, People's Republic of China.
Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
出版信息
Mikrochim Acta. 2019 Nov 19;186(12):805. doi: 10.1007/s00604-019-3926-3.
A method is described for determination and optical imaging of hydrogen peroxide (HO) by using the two-photon (TP) excited fluorescence of silica (SiO) nanoparticles containing Fe(II) ions. In the presence of HO, hydroxyl radicals (•OH) are produced via the Fenton reaction. This leads to quenching of the green fluorescence of a TP-excitable organic dye loaded into the SiONPs. Fluorescence is excited at 370 nm and has an emission peaking at 447 nm. The degree of quenching increases linearly in the 2.5 to 100 μM HO concentration range. The nanoprobe is highly selective and sensitive, with a detection limit of 336 nM. The nanoprobe is biocompatible and was successfully used to image changes in the HO concentration in HeLa cells via TP fluorescence imaging. Graphical abstractSchematic rpresentation of the detection of HO by using the two-photon excited fluorescence of silica nanoparticles (TP-SiONPs) containing Fe. HO triggers the Fenton reaction to produce hydroxyl radicals (•OH), which quench the green fluorescence of the SiONPs.
本文描述了一种利用含有 Fe(II)离子的二氧化硅（SiO）纳米粒子的双光子（TP）激发荧光来测定和光学成像过氧化氢（HO）的方法。在 HO 的存在下，通过芬顿反应产生羟基自由基（•OH）。这导致负载在 SiONP 中的可被 TP 激发的有机染料的绿色荧光猝灭。荧光在 370nm 激发，发射峰在 447nm。在 2.5 至 100μM HO 浓度范围内，猝灭程度呈线性增加。该纳米探针具有高选择性和灵敏度，检测限为 336nM。该纳米探针具有生物相容性，并成功地用于通过 TP 荧光成像来成像 HeLa 细胞中 HO 浓度的变化。
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