经典条件作用诱导的海兔眼内RNA的长期变化。
Prolonged RNA changes in the Hermissenda eye induced by classical conditioning.
作者信息
Nelson T J, Alkon D L
机构信息
Laboratory of Molecular and Cellular Neurobiology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7800-4. doi: 10.1073/pnas.85.20.7800.
Abstract
The incorporation of 32P into mRNA and the total amount of mRNA were increased 3- to 4-fold in eyes isolated from Hermissenda crassicornis trained to associate light with rotation on a turntable compared with animals trained with equal numbers of light and rotation events presented randomly and with naive animals. Incorporation of 32P into poly(A)- RNA was reduced by as much as 60%. The RNA changes were strongly correlated with the degree of learning and could not be accounted for by changes in [32P]ATP content. The RNA changes were maximal at 24 hr and were still detectable after 4 days, indicating that associative conditioning produces a period of increased DNA transcription that could be an intermediate step in memory consolidation. The RNA changes may in part account for recently observed conditioning-specific changes in the synthesis rates of specific proteins.
摘要
与随机呈现相同次数光照和旋转刺激的训练动物以及未受过训练的动物相比，在接受过将光照与转盘旋转相关联训练的肥大海兔（Hermissenda crassicornis）分离出的眼中，32P掺入mRNA的量以及mRNA的总量增加了3至4倍。32P掺入聚（A）-RNA的量减少了多达60%。RNA的变化与学习程度密切相关，且不能用[32P]ATP含量的变化来解释。RNA的变化在24小时时达到最大值，4天后仍可检测到，这表明联合条件作用会产生一段DNA转录增加的时期，这可能是记忆巩固过程中的一个中间步骤。RNA的变化可能部分解释了最近观察到的特定蛋白质合成速率中条件作用特异性的变化。
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