Zhang Yi, Guo Mingcheng, Ma Zhuo, You Chunmei, Gao Xiwu, Shi Xueyan
State Key Lab Breeding Base for Zhejiang Sustainable Plant Pest Control, MOA Key Lab for Pesticide Residue Detection, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Department of Entomology, China Agricultural University, Beijing, 100193, China.
Ecotoxicology. 2020 Jan;29(1):35-44. doi: 10.1007/s10646-019-02125-y. Epub 2019 Nov 20.
Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene MdαE7 in the two strains revealed that four non-synonymous mutations (Trp-Leu, Asp-Glu, Ala-Val, and Ile-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked MdαE7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies.
尽管已有研究提出几种昆虫存在酯酶介导的多杀菌素抗性，但相关分子机制仍知之甚少。在本研究中，我们使用敏感品系（SSS）和多杀菌素抗性近等基因系（N-SRS）研究了家蝇中基于酯酶的多杀菌素抗性机制。结合DEF对N-SRS品系中多杀菌素的增效作用，多杀菌素抗性品系中羧酸酯酶活性降低表明突变酯酶参与了家蝇对多杀菌素的抗性。对两个品系中的羧酸酯酶基因MdαE7进行检测发现，四个非同义突变（Trp-Leu、Asp-Glu、Ala-Val和Ile-Val）可能与家蝇对多杀菌素的抗性有关。单核苷酸多态性分析进一步表明这四个突变与多杀菌素抗性之间存在密切关系。此外，定量实时PCR揭示了N-SRS品系中MdαE7的雌性关联表达模式，这可能导致家蝇对多杀菌素产生性别差异抗性。
