Asadi Paniza, Mohebbi Seyed Reza, Hosseini Seyed Masoud, Zali Mohammad Reza
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2019 Fall;12(4):309-314.
This study aimed to evaluate rs1179251 single nucleotide polymorphism in the IL-22 gene as a host factor and its effect on chronic hepatitis B infection.
Interleukin 22 (IL-22) belongs to a group of recently discovered cytokines, and it is produced and secreted by innate lymphoid cells (ILCs) and T helper 22 (Th22) cells. This cytokine plays dual roles as pro-inflammatory and anti-inflammatory effects in various conditions and different tissues of the body.
This study was performed based on a case-control format to assess IL-22 rs1179251 single nucleotide polymorphism genotypic and allelic frequencies among 227 hepatitis B chronic patients and 227 healthy controls. The polymerase chain reaction and restriction fragment length polymorphism techniques were employed to determine the polymorphism's genotypes.
Genotypes Frequencies in patients' group were determined CC 59.91%, CG 37.89%, and GG 2.20% respectively in comparison to CC 63.44%, CG 31.72% and GG 4.85% in control group. The findings revealed that there was no statistically significant difference in the genotypes (P=0.156) frequencies of IL-22 gene polymorphism (rs1179251) between patients and control groups.
No association was found between rs1179251 single nucleotide polymorphism in IL-22 gene and chronic hepatitis B infection. So, in spite of the importance of IL-22 gene in immune responses, the studied polymorphism does not serve a decisive role in susceptibility to hepatitis B virus chronic infection.
本研究旨在评估白细胞介素-22（IL-22）基因中的rs1179251单核苷酸多态性作为宿主因素及其对慢性乙型肝炎感染的影响。
白细胞介素22（IL-22）属于一组最近发现的细胞因子，由天然淋巴细胞（ILC）和辅助性T细胞22（Th22）产生并分泌。这种细胞因子在身体的各种条件和不同组织中发挥促炎和抗炎的双重作用。
本研究采用病例对照形式，评估227例慢性乙型肝炎患者和227例健康对照者中IL-22 rs1179251单核苷酸多态性的基因型和等位基因频率。采用聚合酶链反应和限制性片段长度多态性技术确定多态性的基因型。
患者组基因型频率分别为CC 59.91%、CG 37.89%和GG 2.20%，而对照组分别为CC 63.44%、CG 31.72%和GG 4.85%。研究结果显示，患者组和对照组之间IL-22基因多态性（rs1179251）的基因型频率无统计学显著差异（P=0.156）。
未发现IL-22基因中的rs1179251单核苷酸多态性与慢性乙型肝炎感染之间存在关联。因此，尽管IL-22基因在免疫反应中很重要，但所研究的多态性在乙型肝炎病毒慢性感染易感性方面不起决定性作用。
