Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran.
Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran.
Int J Biol Macromol. 2020 Jun 1;152:1089-1097. doi: 10.1016/j.ijbiomac.2019.10.197. Epub 2019 Nov 18.
This research investigated the colonization efficiency and survival rate of nanoencapsulated Bacillus subtilis Vru1 prepared with sodium alginate (NaAlg), starch and bentonite and, their efficiency for controlling Rhizoctonia solani. The XRD and FTIR analysis in this research indicated the absence of chemical reactions, and correct mixing efficiency was funded with alginate, bentonite and starch. The highest release of B. subtilis Vru1 was 4 × 10 in 45th day of storage. The quantity of colonization by B. subtilis Vru1 nanocapsules was lower than that of free B. subtilis Vru1 at days 5-20 and was significantly higher than that of free B. subtilis VRU1 and encapsulated B. subtilis VRU1 without Titanium dioxide nanoparticle, after day 35. This level was maintained for up to 45th day. The results of this experiments indicated that the highest and lowest inhibition of R. solani on bean observed in Vru1 nanocapsules with 90% and free Vru1 with 60% respectively. and Vru1 capsule without titanium nanoparticles (TNs) decreased the disease by 75%. The nanoencapsulated B. subtilis Vru1 strain significantly increased the bean vegetative growth parameters. This finding was probably attributed to the enhancement in the number of the bacterium and the high level of metabolite production such as indole-3-acetic acid. Thus, nanocapsule formulation is a potential alternative for sustainable agriculture.
本研究调查了用海藻酸钠（NaAlg）、淀粉和膨润土制备的纳米包封枯草芽孢杆菌 Vru1 的定植效率和存活率，以及它们控制立枯丝核菌的效率。本研究中的 XRD 和 FTIR 分析表明没有化学反应，并且正确的混合效率是用海藻酸钠、膨润土和淀粉实现的。B. subtilis Vru1 的最高释放量为 4×10 在第 45 天的储存。B. subtilis Vru1 纳米胶囊的定植数量在第 5-20 天低于游离 B. subtilis Vru1，在第 35 天后明显高于游离 B. subtilis VRU1 和无二氧化钛纳米颗粒的包封 B. subtilis VRU1。这一水平一直保持到第 45 天。实验结果表明，Vru1 纳米胶囊对豆类立枯丝核菌的抑制作用最高，为 90%，游离 Vru1 的抑制作用最低，为 60%，而无钛纳米粒子（TNs）的 Vru1 胶囊则降低了 75%的病害。纳米包封枯草芽孢杆菌 Vru1 株显著增加了豆类的营养生长参数。这一发现可能归因于细菌数量的增加和代谢物如吲哚-3-乙酸的高水平产生。因此，纳米胶囊配方是可持续农业的一种潜在替代方法。
