Rodríguez-Padilla M L, de la Fuente M, Bellido C, Aguilar E, Aguilar R
Departamento de Fisiología, Facultad de Medicina, Cordoba, España.
Rev Esp Fisiol. 1988 Mar;44(1):63-8.
The body growth and the onset of puberty in spontaneously hypertensive rats (SHR) and in normotensive controls (WKY) have been studied. In female rats the onset of puberty was determined by both the age and the body weight at which the vaginal opening and first estrus appeared, as well as the ability of estradiol and progesterone to induce pituitary LH release. For this purpose females were injected with estradiol benzoate (0.1 mg/kg) and progesterone (1 mg/rat). Control animals received only oil vehicle. In male rats, puberty was assessed by studying the age and body weight at the time of balano-preputial separation. In another experiment, SH and WKY rats were decapitated on day 30 to determine FSH, LH, PRL, GH and testosterone plasma levels in males and FSH and LH in females. The results obtained show: a) A greater body weight, at all the ages studied (every 4 days between days 28 and 92) in SHR animals. b) A delay in vaginal opening and first estrus presentation in SHR females. c) Absence of spontaneous LH peaks in WKY females. d) Advancement in balano-preputial separation in SHR males and e) Higher plasma FSH levels in SHR males than in WKY males, without differences in other hormones.
对自发性高血压大鼠（SHR）和正常血压对照大鼠（WKY）的身体生长和青春期启动进行了研究。在雌性大鼠中，青春期启动由阴道开口和首次发情出现时的年龄和体重以及雌二醇和孕酮诱导垂体促黄体生成素（LH）释放的能力来确定。为此，给雌性大鼠注射苯甲酸雌二醇（0.1mg/kg）和孕酮（1mg/只）。对照动物仅接受油性载体。在雄性大鼠中，通过研究龟头-包皮分离时的年龄和体重来评估青春期。在另一项实验中，在第30天将SHR和WKY大鼠断头，以测定雄性大鼠的促卵泡生成素（FSH）、LH、催乳素（PRL）、生长激素（GH）和睾酮血浆水平以及雌性大鼠的FSH和LH。获得的结果显示：a）在所有研究年龄（第28天至92天期间每4天），SHR动物的体重更大。b）SHR雌性大鼠阴道开口和首次发情出现延迟。c）WKY雌性大鼠无自发性LH峰值。d）SHR雄性大鼠龟头-包皮分离提前，以及e）SHR雄性大鼠的血浆FSH水平高于WKY雄性大鼠，其他激素无差异。
