College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Key Laboratory of Food Quality and Safety, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu Province, China.
Virol J. 2019 Nov 21;16(1):141. doi: 10.1186/s12985-019-1228-3.
Rice stripe virus (RSV) causes one of the most important rice virus diseases of plants in East Asia. However, the molecular mechanisms controlling rice resistance to RSV infection are largely unknown. Recently, several studies presented a novel model that melatonin (MT) and nitric oxide (NO) participate in the plant-pathogen interaction in a synergetic manner. In this study, there was a difference in MT content between two rice varieties that correlated with one being susceptible and one being resistant to RSV, which suggested that MT is related to RSV resistance. In addition, a test with two NO biosynthesis inhibitors revealed that NO inhibitor were able to increase the disease incidence of RSV. A pharmacological experiment with exogenous MT and NO showed that increased MT and NO in the MT-pretreated plants led to lower disease incidences; however, only NO increased in a NO-releasing reagent [sodium nitroprusside (SNP)] pretreated plants. The expressions level of OsPR1b and OsWRKY 45 were significantly induced by MT and NO. These results suggest that rice resistance to RSV can be improved by increased MT through a NO-dependent pathway.
水稻条纹病毒（RSV）是东亚地区植物最重要的病毒病之一。然而，控制水稻对 RSV 感染的抗性的分子机制在很大程度上尚不清楚。最近，几项研究提出了一个新模型，即褪黑素（MT）和一氧化氮（NO）以协同方式参与植物-病原体相互作用。在这项研究中，两种水稻品种之间 MT 含量存在差异，与一种对 RSV 敏感和一种对 RSV 抗性相关，这表明 MT 与 RSV 抗性有关。此外，用两种 NO 生物合成抑制剂进行的测试表明，NO 抑制剂能够增加 RSV 的发病率。用外源 MT 和 NO 进行的药理学实验表明，在 MT 预处理的植物中增加 MT 和 NO 会导致较低的发病率；然而，只有在用一氧化氮供体[硝普钠（SNP）]预处理的植物中才会增加 NO。MT 和 NO 显著诱导 OsPR1b 和 OsWRKY45 的表达水平。这些结果表明，通过增加 MT 可以通过依赖于 NO 的途径来提高水稻对 RSV 的抗性。
