New York University Langone Health, New York, NY, USA.
New York Genome Center, New York, NY, USA.
Genome Biol. 2019 Nov 21;20(1):248. doi: 10.1186/s13059-019-1853-6.
Activation of regulatory elements is thought to be inversely correlated with DNA methylation levels. However, it is difficult to determine whether DNA methylation is compatible with chromatin accessibility or transcription factor (TF) binding if assays are performed separately. We developed a fast, low-input, low sequencing depth method, EpiMethylTag, that combines ATAC-seq or ChIP-seq (M-ATAC or M-ChIP) with bisulfite conversion, to simultaneously examine accessibility/TF binding and methylation on the same DNA. Here we demonstrate that EpiMethylTag can be used to study the functional interplay between chromatin accessibility and TF binding (CTCF and KLF4) at methylated sites.
调控元件的激活被认为与 DNA 甲基化水平呈负相关。然而，如果单独进行检测，就很难确定 DNA 甲基化是否与染色质可及性或转录因子（TF）结合兼容。我们开发了一种快速、低输入、低测序深度的方法 EpiMethylTag，它将 ATAC-seq 或 ChIP-seq（M-ATAC 或 M-ChIP）与亚硫酸氢盐转化相结合，可同时在同一 DNA 上检测到可及性/TF 结合和甲基化。在这里，我们证明 EpiMethylTag 可用于研究甲基化位点处染色质可及性和 TF 结合（CTCF 和 KLF4）之间的功能相互作用。
