Yamada K, Neumann H A, Fiebig H H, Engelhardt R, Tokita H
Medizinische Klinik, Albert-Ludwigs-Universität, Freiburg, FRG.
Recent Results Cancer Res. 1988;109:250-7. doi: 10.1007/978-3-642-83263-5_27.
In order to estimate its ability to predict the thermochemosensitivity of human cancers, a rapid in vitro assay based on morphological changes in the nucleus was performed on eight different human tumors (four malignant melanomas, two lung tumors, one renal carcinoma, and leukemia K-562). Nude mice, implanted with tumors, supplied the tumor material, with the exception of leukemia. Nimustine, melphalan, mitomycin C, vincristine and vinblastine were tested. Tumor cells developed karyorrhectic changes after incubation for 4 h with each of the aforementioned five drugs. An increase in the karyorrhectic changes was observed with hyperthermia at 43 degrees C. The individual tumors showed different sensitivities to 43 degrees C. Five of the eight tumors were significantly sensitive to 43 degrees C. However, in two thermosensitive tumors no drug enhancement was recognized at 43 degrees C. In four tumors several drugs were synergistically enhanced by hyperthermia at 43 degrees C. This study suggests that this simple method may be of clinical use in predicting response to thermochemotherapy.
为了评估其预测人类癌症热化学敏感性的能力，对八种不同的人类肿瘤（四例恶性黑色素瘤、两例肺癌、一例肾癌和白血病K - 562）进行了一项基于细胞核形态变化的快速体外试验。除白血病外，植入肿瘤的裸鼠提供肿瘤材料。对尼莫司汀、美法仑、丝裂霉素C、长春新碱和长春碱进行了测试。将肿瘤细胞与上述五种药物中的每一种孵育4小时后，出现了核固缩变化。在43℃热疗时，核固缩变化增加。各个肿瘤对43℃显示出不同的敏感性。八个肿瘤中有五个对43℃显著敏感。然而，在两个热敏性肿瘤中，在43℃未观察到药物增强作用。在四个肿瘤中，几种药物在43℃热疗时协同增强。这项研究表明，这种简单方法可能在预测热化疗反应方面具有临床应用价值。
