Yamada K, Neumann H A, Fiebig H H, Engelhardt R, Tokita H
Medizinische Klinik, Albert-Ludwigs-Universität, Freiburg, FRG.
Recent Results Cancer Res. 1988;107:177-83. doi: 10.1007/978-3-642-83260-4_25.
We previously reported a rapid in vitro assay based on morphological changes in the nucleus in order to predict response to thermochemotherapy. It was strongly suggested that this simple method may be clinically useful. In the present study, a comparison with the clonogenic assay was carried out on eight different human tumors (three malignant melanomas, two lung carcinomas, two colon carcinomas and one leukemia). Melphalan, mitomycin C and vincristine were tested. Correlation between the two test systems was dependent upon the criteria for each test system. At the level of less than 50% survival of colony as compared with normothermic dishes in clonogenic assay, there was a high correlation between the two test systems for sensitive tumor to 43 degrees C. In respect of response to thermochemotherapy, when only karyorrhexis changes in the nucleus were selected as an activity criteria in our cytotoxic test, parallel data between the two test systems were obtained.
我们之前报道了一种基于细胞核形态变化的快速体外检测方法，用于预测热化疗反应。强烈提示这种简单方法可能具有临床实用性。在本研究中，对8种不同的人类肿瘤（3例恶性黑色素瘤、2例肺癌、2例结肠癌和1例白血病）进行了与克隆形成试验的比较。测试了美法仑、丝裂霉素C和长春新碱。两种检测系统之间的相关性取决于每个检测系统的标准。在克隆形成试验中，与常温培养皿相比，集落存活率低于50%的水平时，对于对43℃敏感的肿瘤，两种检测系统之间存在高度相关性。就热化疗反应而言，当在我们的细胞毒性试验中仅选择细胞核核碎裂变化作为活性标准时，获得了两种检测系统之间的平行数据。
