Institute of Low Temperature and Structure Research, PAS, ul. Okolna 2, PL-50-422, Wroclaw, Poland.
Institute of Genetics and Microbiology, Wroclaw University, Przybyszewskiego 63, 51-148, Wroclaw, Poland.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110295. doi: 10.1016/j.msec.2019.110295. Epub 2019 Oct 10.
Hexagonal nanocrystalline powders of the non-doped Ca(PO)(OH) as well as activated with Ag and Eu ions were synthesized by using different wet chemistry methods. Moreover, the obtained hydroxyapatite was loaded with Ag, as well as nitroimidazole antimicrobials: metronidazole and tinidazole. The structural properties of the products were analyzed by X-ray diffraction (XRD), scanning (SEM) and transmission (TEM) electron microscopy as well as infrared (IR) and Raman spectroscopy. The photoluminescence properties of the Eu and Ag co-doped Ca(PO)(OH) were characterized via the PL emission, excitation spectra and the luminescence decay curve. The antimicrobial activity of the obtained materials against Prevotella bivia and Parabacteroides distasonis was studied. The cytotoxicity assessment was carried out on the human osteosarcoma cell line (U2OS) as well as human red blood cells (RBC). The choice of the in vitro model was based on the fact that U2OS is a cancer cell line derived from bone tissue which is rich in apatites that play a pivotal role in the extracellular matrix formation. RBCs are the most abundant blood cells and they are used as a cell model in the study of biocompatibility of new prepared biocompounds with potential medical applications. The obtained multifunctional materials do not exhibit the haemolytic activity, therefore, they could be used as a promising antimicrobial agent and for anaerobic bacteria.
采用不同的湿化学方法合成了未掺杂的 Ca(PO)(OH)以及用 Ag 和 Eu 离子激活的六方纳米晶粉末。此外，还将获得的羟基磷灰石负载了 Ag 以及硝基咪唑类抗生素：甲硝唑和替硝唑。通过 X 射线衍射（XRD）、扫描（SEM）和透射（TEM）电子显微镜以及红外（IR）和拉曼光谱分析了产物的结构特性。通过 PL 发射、激发光谱和荧光衰减曲线对 Eu 和 Ag 共掺杂 Ca(PO)(OH)的光致发光性能进行了表征。研究了所得材料对 Prevotella bivia 和 Parabacteroides distasonis 的抗菌活性。在人骨肉瘤细胞系（U2OS）和人红细胞（RBC）上进行了细胞毒性评估。体外模型的选择基于以下事实：U2OS 是一种源自富含磷灰石的骨组织的癌细胞系，磷灰石在细胞外基质形成中起着关键作用。RBC 是最丰富的血细胞，它们被用作研究具有潜在医学应用的新制备的生物化合物的生物相容性的细胞模型。所得的多功能材料不具有溶血活性，因此可作为有前途的抗菌剂和用于治疗厌氧菌。
