Department of Materials Science and Engineering, Jinan University, Guangzhou, 510632, China.
Department of Materials Science and Engineering, Jinan University, Guangzhou, 510632, China.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110282. doi: 10.1016/j.msec.2019.110282. Epub 2019 Oct 7.
Three-dimensional graphene materials have been widely studied in many fields for their role as potential absorbent, especially for bilirubin adsorption. In this study, we developed a simple method to prepare reduced graphene aerogel beads as hemoperfusion materials for fast bilirubin adsorption. The graphene oxide (GO) aerogel beads were produced by self-assembly of GO nanosheet that cross-linked by Ca previously in a coagulation bath, then it was reduced by ascorbic acid and lyophilized to yield the reduced graphene aerogel beads. The beads had a regular macroscopic spherical structure with a diameter of about 1.3-2 mm, where the macroporosity was about 10 μm and the mesoporosity was about 12 nm. The macro-mesoporous structure also gave the reduced graphene aerogel beads ultra-high mechanical strengths and high specific surface area, which were both important for hemoperfusion materials. Moreover, the fixed-bed column adsorption revealed that the reduced graphene aerogel beads manifested excellent bilirubin adsorption (649.512 mg/g) with a rapid adsorption equilibrium time (1.5 h) under the optimized conditions. Even in the bilirubin-enriched blood, the adsorption capacity of the beads could reach 367.14 mg/g. Furthermore, the aerogel beads had a low hemolysis ratio and improved anticoagulant property showing good blood compatibility. Hence, the spherical reduced graphene aerogel beads with millimeter-level size presented a good potential for clinical applications in hemoperfusion therapy.
三维石墨烯材料因其作为潜在吸附剂的作用而在许多领域得到了广泛研究，尤其是在胆红素吸附方面。在这项研究中，我们开发了一种简单的方法来制备还原氧化石墨烯气凝胶珠作为血液灌流材料，用于快速吸附胆红素。氧化石墨烯气凝胶珠是通过 GO 纳米片在先前的凝固浴中通过 Ca 交联自组装而成的，然后用抗坏血酸还原并冻干得到还原氧化石墨烯气凝胶珠。这些珠子具有规则的宏观球形结构，直径约为 1.3-2mm，其中大孔约为 10μm，中孔约为 12nm。宏观-介孔结构还赋予了还原氧化石墨烯气凝胶珠超高的机械强度和高比表面积，这对于血液灌流材料都是非常重要的。此外，固定床柱吸附表明，在优化条件下，还原氧化石墨烯气凝胶珠表现出优异的胆红素吸附能力（649.512mg/g），吸附平衡时间非常快（1.5h）。即使在胆红素富集的血液中，珠子的吸附能力也可以达到 367.14mg/g。此外，气凝胶珠具有较低的溶血率和改善的抗凝性能，显示出良好的血液相容性。因此，具有毫米级尺寸的球形还原氧化石墨烯气凝胶珠在血液灌流治疗的临床应用中具有良好的应用前景。
