Li Yingying, Xie Haihuan, Wang Jin, Li Xiangmei, Xiao Zhili, Xu Zhenlin, Lei Hongtao, Shen Xing
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
Foods. 2021 Aug 30;10(9):2041. doi: 10.3390/foods10092041.
In recent years, furosemide has been found to be abused in slimming health foods. There is an urgent need for a simpler, faster method for detecting furosemide in slimming health foods. In this study, a rapid, convenient and sensitive lateral flow immunochromatography (LFIA) based on Au nanoparticles (AuNPs) was established for the first time. Under optimal conditions, the qualitative limit of detection (LOD) of the AuNPs-based LFIA was 1.0~1.2 μg/g in slimming health foods with different substrates. AuNPs-LFIA could specifically detect furosemide within 12 min (including sample pretreatment) and be read by the naked eye. The developed AuNPs-LFIA showed high consistency with liquid chromatography with tandem mass spectrometry (LC-MS/MS), and no false positive or false negative results were found in spiked slimming health foods, proving that the AuNPs-LFIA should be accurate and reliable. The AuNPs-LFIA reported here provides a serviceable analytical tool for the on-site detection and rapid initial screening of furosemide for the first time.
近年来，发现呋塞米在减肥保健食品中被滥用。迫切需要一种更简单、快速的方法来检测减肥保健食品中的呋塞米。本研究首次建立了基于金纳米粒子（AuNPs）的快速、便捷、灵敏的侧向流动免疫色谱法（LFIA）。在最佳条件下，基于AuNPs的LFIA在不同基质的减肥保健食品中的定性检测限（LOD）为1.0~1.2 μg/g。AuNPs-LFIA可在12分钟内（包括样品预处理）特异性检测呋塞米，且可肉眼读取结果。所开发的AuNPs-LFIA与液相色谱-串联质谱法（LC-MS/MS）具有高度一致性，在添加了呋塞米的减肥保健食品中未发现假阳性或假阴性结果，证明AuNPs-LFIA准确可靠。本文报道的AuNPs-LFIA首次为呋塞米的现场检测和快速初步筛查提供了一种实用的分析工具。
