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压力和分散剂对从墨西哥湾深海沉积物中新分离出的红球菌菌株石油生物降解的影响

Influence of pressure and dispersant on oil biodegradation by a newly isolated Rhodococcus strain from deep-sea sediments of the gulf of Mexico.

作者信息

Hackbusch Steffen, Noirungsee Nuttapol, Viamonte Juan, Sun Xiaoxu, Bubenheim Paul, Kostka Joel E, Müller Rudolf, Liese Andreas

机构信息

Institute of Technical Biocatalysis, Hamburg University of Technology (TUHH), Hamburg, Germany.

Georgia Institute of Technology School of Biology and Earth & Atmospheric Sciences, Atlanta, GA, USA.

出版信息

Mar Pollut Bull. 2020 Jan;150:110683. doi: 10.1016/j.marpolbul.2019.110683. Epub 2019 Nov 18.

DOI:10.1016/j.marpolbul.2019.110683
PMID:31753565
Abstract

A new Rhodococcus strain, capable of degrading crude oil, was isolated from the Gulf of Mexico deep-sea sediment and was investigated for its biodegradation characteristics under atmospheric as well as under deep-sea pressure (1500 m = 15 MPa). Additionally, the effect of dispersant (Corexit EC9500A) addition was studied. Rhodococcus sp. PC20 was shown to degrade 60.5 ± 10.7% of the saturated and aromatic fraction of crude oil at atmospheric pressure and 74.2 ± 9.1% at deep-sea level pressure within 96 h. Degradation rates, especially for monoaromatic hydrocarbons, were significantly higher at elevated pressure compared to atmospheric pressure. This study found a growth inhibiting effect at a dispersant to oil ratio of 1:100 and higher. This effect of the dispersant was enhanced when elevated pressure was applied.

摘要

从墨西哥湾深海沉积物中分离出一种能够降解原油的新型红球菌菌株,并对其在常压和深海压力(1500米 = 15兆帕)下的生物降解特性进行了研究。此外,还研究了添加分散剂(Corexit EC9500A)的影响。结果表明,红球菌PC20在常压下96小时内可降解原油饱和及芳烃馏分的60.5±10.7%,在深海压力下可降解74.2±9.1%。与常压相比,高压下的降解速率,尤其是单芳烃的降解速率显著更高。本研究发现,分散剂与油的比例为1:100及以上时具有生长抑制作用。施加高压时,分散剂的这种作用会增强。

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