Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2, Beijing 100193, China.
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Sci Total Environ. 2023 Nov 1;897:165287. doi: 10.1016/j.scitotenv.2023.165287. Epub 2023 Jul 6.
Chemical pollution and global warming are two major threats to reptiles, and these two factors can interact with each other. Glyphosate have attracted worldwide attention due to their ubiquitous occurrence, yet their impact on reptiles remains unknown. We designed a crossover experiment with different external GBH exposures (control/GBH) x different environmental temperatures (current climate treatment/warmer climate treatment) over 60 days to simulate environmental exposure in the Mongolian Racerunner lizard (Eremias argus). Preferred body temperature and active body temperature data were collected to calculate the accuracy of thermoregulation, while liver detoxification metabolic enzymes, oxidative stress system function, and the non-targeted metabolome of the brain tissue were assessed. Warmer-treated lizards adjusted their physiological levels and behavioral strategies in response to increased ambient temperatures and maintained body temperature homeostasis at moderate thermal perturbations. GBH-treated lizards suffered from oxidative damage to the brain tissue and abnormal histidine metabolism, thus their thermoregulatory accuracy reduced. Interestingly, at elevated ambient temperatures, GBH treatment did not affect on their thermoregulatory, possibly through several temperature-dependent detoxification mechanisms. Importantly, this data suggested that the subtle toxicological effects of GBH may threaten increasingly thermoregulation behavior of E. argus with species-wide repercussions, as climate change and exposure time extension.
化学污染和全球变暖是爬行动物面临的两大威胁，这两个因素可以相互作用。草甘膦由于其普遍存在而引起了全球关注，但它们对爬行动物的影响仍不清楚。我们设计了一个交叉实验，在 60 天内用不同的外部 GBH 暴露（对照/GBH）x 不同的环境温度（当前气候处理/较暖气候处理）来模拟蒙古沙蜥（Eremias argus）的环境暴露。收集了首选体温和活动体温数据来计算体温调节的准确性，同时评估了肝脏解毒代谢酶、氧化应激系统功能和脑组织的非靶向代谢组。较暖处理的蜥蜴通过增加环境温度来调整其生理水平和行为策略，并在中等热干扰下维持体温平衡。GBH 处理的蜥蜴遭受脑组织氧化损伤和组氨酸代谢异常，因此其体温调节准确性降低。有趣的是，在较高的环境温度下，GBH 处理不会影响它们的体温调节，这可能是通过几种依赖温度的解毒机制。重要的是，这些数据表明，草甘膦的细微毒性可能会威胁到 E. argus 日益增加的体温调节行为，因为气候变化和暴露时间的延长。
