Research Laboratory of Spectroscopy & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, Iran; Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Research Laboratory of Spectroscopy & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, Iran.
Food Chem. 2020 May 1;311:125876. doi: 10.1016/j.foodchem.2019.125876. Epub 2019 Nov 11.
In this research, a new design of channels in a lab-on-a-chip device with flat electromembrane extraction (LOC-FLEME) was fabricated. The latter microfluidic device was successfully used for the determination of 2-amino-3-methyl imidazo[4-5-f]quinolone (IQ), 2-amino-3, 8-dimethlylimidazo[4, 5-f]quinolone (MeIQ), 2-amino-3,4- dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6-phenylimidazo[4, 5-b] pyridine (PhIP) in grilled meat, by on-line coupling of LOC-FLEME to an HPLC system. Important parameters in extraction process were optimized. The calibration curve was linear over the range of 5-1000 ng g with a correlation coefficient higher than 0.9991. The relative recoveries were between 95 and 98% at three concentration levels. The relative standard deviations were 4.1 to 6.0%. The limits of detection were 1.4, 0.9, 1.7 and 1.3 ng g for PhIP, IQ, MeIQ and MeIQx, respectively. Sums of 4HAAs concentrations for different grilled meat samples were 2.75-6.17 ng g.
在这项研究中，设计了一种带有平面电膜萃取（LOC-FLEME）的微流控芯片通道。成功地将这种微流控装置用于在线耦合 LOC-FLEME 与 HPLC 系统，以测定烤肉类中的 2-氨基-3-甲基咪唑并[4,5-f]喹啉（IQ）、2-氨基-3,8-二甲基咪唑并[4,5-f]喹啉（MeIQ）、2-氨基-3,4-二甲基咪唑并[4,5-f]喹喔啉（MeIQx）和 2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）。优化了萃取过程中的重要参数。校准曲线在 5-1000ng/g 范围内呈线性，相关系数高于 0.9991。在三个浓度水平下，相对回收率在 95%至 98%之间。相对标准偏差为 4.1%至 6.0%。PhIP、IQ、MeIQ 和 MeIQx 的检出限分别为 1.4、0.9、1.7 和 1.3ng/g。不同烤肉类样品中 4HAAs 的浓度总和为 2.75-6.17ng/g。
